The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs

Hua Deng, Sean Cahill, Linda Kurz, Robert Callender

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.

Original languageEnglish (US)
Pages (from-to)1952-1953
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number7
DOIs
Publication statusPublished - Feb 25 2004

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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