The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs

Hua Deng, Sean M. Cahill, Linda Kurz, Robert Callender

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.

Original languageEnglish (US)
Pages (from-to)1952-1953
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number7
DOIs
StatePublished - Feb 25 2004

Fingerprint

Enzyme Inhibitors
Protons
Enzymes
Adenosine Deaminase
Hydrogen
nebularine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

@article{15fc99c6c9f84332bc4ff41aa24da8cb,
title = "The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs",
abstract = "We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.",
author = "Hua Deng and Cahill, {Sean M.} and Linda Kurz and Robert Callender",
year = "2004",
month = "2",
day = "25",
doi = "10.1021/ja0378714",
language = "English (US)",
volume = "126",
pages = "1952--1953",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - The Assignment of Downfield Proton Resonances in an Enzyme Inhibitor Complex Using Time-Dependent Saturation Transferred NOEs

AU - Deng, Hua

AU - Cahill, Sean M.

AU - Kurz, Linda

AU - Callender, Robert

PY - 2004/2/25

Y1 - 2004/2/25

N2 - We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.

AB - We have used time-dependent saturation transferred NOE (STNOE) measurements to assign two downfield resonances in the proton spectrum of an adenosine deaminase-purine riboside mixture. Our results show that this method can be used to detect structural changes that occur upon inhibitor binding to the enzyme and to determine which protons of the bound inhibitor are strongly hydrogen bonded in the complex.

UR - http://www.scopus.com/inward/record.url?scp=1242297056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1242297056&partnerID=8YFLogxK

U2 - 10.1021/ja0378714

DO - 10.1021/ja0378714

M3 - Article

C2 - 14971925

AN - SCOPUS:1242297056

VL - 126

SP - 1952

EP - 1953

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 7

ER -