The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome

Li qun Fang, Pan he Zhang, Bao an Yang, Xiao ming Wu, Qiu min Zhao, Wei Liu, Hong Liu, Yong qiang Deng, Lin Zhan, Wei guo Han, Fu shuang Lu, Jin song Wu, Hong Yang, Qing yu Zhu, Wu chun Cao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

OBJECTIVE: To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS. METHODS: Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire. RESULTS: The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%. CONCLUSION: IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.

Original languageEnglish (US)
Pages (from-to)484-486
Number of pages3
JournalZhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
Volume24
Issue number6
StatePublished - 2003
Externally publishedYes

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Severe Acute Respiratory Syndrome
Fluorescence
Immunoglobulin M
Immunoglobulin G
Coronavirus
SARS Virus
Antibodies
Chi-Square Distribution
Serum
Sensitivity and Specificity

Cite this

Fang, L. Q., Zhang, P. H., Yang, B. A., Wu, X. M., Zhao, Q. M., Liu, W., ... Cao, W. C. (2003). The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome. Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 24(6), 484-486.

The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome. / Fang, Li qun; Zhang, Pan he; Yang, Bao an; Wu, Xiao ming; Zhao, Qiu min; Liu, Wei; Liu, Hong; Deng, Yong qiang; Zhan, Lin; Han, Wei guo; Lu, Fu shuang; Wu, Jin song; Yang, Hong; Zhu, Qing yu; Cao, Wu chun.

In: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, Vol. 24, No. 6, 2003, p. 484-486.

Research output: Contribution to journalArticle

Fang, LQ, Zhang, PH, Yang, BA, Wu, XM, Zhao, QM, Liu, W, Liu, H, Deng, YQ, Zhan, L, Han, WG, Lu, FS, Wu, JS, Yang, H, Zhu, QY & Cao, WC 2003, 'The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome', Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, vol. 24, no. 6, pp. 484-486.
Fang, Li qun ; Zhang, Pan he ; Yang, Bao an ; Wu, Xiao ming ; Zhao, Qiu min ; Liu, Wei ; Liu, Hong ; Deng, Yong qiang ; Zhan, Lin ; Han, Wei guo ; Lu, Fu shuang ; Wu, Jin song ; Yang, Hong ; Zhu, Qing yu ; Cao, Wu chun. / The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome. In: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi. 2003 ; Vol. 24, No. 6. pp. 484-486.
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abstract = "OBJECTIVE: To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS. METHODS: Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire. RESULTS: The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1{\%} and 16.3{\%} respectively and then increased up to 89.8{\%} for IgG and 65.3{\%} for IgM. After 25 days of the onset of the disease, 90.9{\%} patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90{\%}. CONCLUSION: IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.",
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AU - Zhang, Pan he

AU - Yang, Bao an

AU - Wu, Xiao ming

AU - Zhao, Qiu min

AU - Liu, Wei

AU - Liu, Hong

AU - Deng, Yong qiang

AU - Zhan, Lin

AU - Han, Wei guo

AU - Lu, Fu shuang

AU - Wu, Jin song

AU - Yang, Hong

AU - Zhu, Qing yu

AU - Cao, Wu chun

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N2 - OBJECTIVE: To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS. METHODS: Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire. RESULTS: The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%. CONCLUSION: IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.

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