The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome

Li qun Fang, Pan he Zhang, Bao an Yang, Xiao ming Wu, Qiu min Zhao, Wei Liu, Hong Liu, Yong qiang Deng, Lin Zhan, Wei guo Han, Fu shuang Lu, Jin song Wu, Hong Yang, Qing yu Zhu, Wu chun Cao

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Abstract

OBJECTIVE: To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS. METHODS: Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire. RESULTS: The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%. CONCLUSION: IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.

Original languageEnglish (US)
Pages (from-to)484-486
Number of pages3
JournalZhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi
Volume24
Issue number6
Publication statusPublished - Jun 2003

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fang, L. Q., Zhang, P. H., Yang, B. A., Wu, X. M., Zhao, Q. M., Liu, W., ... Cao, W. C. (2003). The application of indirect immuno-fluorescence assay in the diagnosis of severe acute respiratory syndrome. Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 24(6), 484-486.