The acute effects of acrylamide on astrocyte functions

Michael Aschner; Cheng Chen Cao; Qi Wu; Marvin A. Friedman

doi:10.1111/j.1749-6632.2003.tb07537.x

The acute effects of acrylamide on astrocyte functions

Michael Aschner, Cheng Chen Cao, Qi Wu, Marvin A. Friedman

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We assessed biochemical endpoints indicative of acute toxicity in neonatal rat primary astrocyte cultures exposed to acrylamide. Metallothionein (MT), glutamine synthetase (GS), glutamate/aspartate transporter (GLAST), and taurine transporter (tau-T) mRNA expression levels as well as cell volume were determined in astrocytes acutely treated with 0.1 and 1.0 mM acrylamide. Statistically significant changes in acrylamide treated astrocytes were noted for GS (0.1 mM) and GLAST (1.0 mM) mRNA expression levels. All other measurements were insignificant in comparison with controls, suggesting that astrocytic function is minimally compromised even at exceedingly high levels of acute acrylamide exposure.

Original language	English (US)
Pages (from-to)	296-304
Number of pages	9
Journal	Annals of the New York Academy of Sciences
Volume	993
DOIs	https://doi.org/10.1111/j.1749-6632.2003.tb07537.x
State	Published - 2003
Externally published	Yes

Keywords

Acrylamide
Astrocyte function
Glutamate
Metallothionein

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
History and Philosophy of Science

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10.1111/j.1749-6632.2003.tb07537.x

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title = "The acute effects of acrylamide on astrocyte functions",

abstract = "We assessed biochemical endpoints indicative of acute toxicity in neonatal rat primary astrocyte cultures exposed to acrylamide. Metallothionein (MT), glutamine synthetase (GS), glutamate/aspartate transporter (GLAST), and taurine transporter (tau-T) mRNA expression levels as well as cell volume were determined in astrocytes acutely treated with 0.1 and 1.0 mM acrylamide. Statistically significant changes in acrylamide treated astrocytes were noted for GS (0.1 mM) and GLAST (1.0 mM) mRNA expression levels. All other measurements were insignificant in comparison with controls, suggesting that astrocytic function is minimally compromised even at exceedingly high levels of acute acrylamide exposure.",

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T1 - The acute effects of acrylamide on astrocyte functions

AU - Aschner, Michael

AU - Cao, Cheng Chen

AU - Wu, Qi

AU - Friedman, Marvin A.

PY - 2003

Y1 - 2003

N2 - We assessed biochemical endpoints indicative of acute toxicity in neonatal rat primary astrocyte cultures exposed to acrylamide. Metallothionein (MT), glutamine synthetase (GS), glutamate/aspartate transporter (GLAST), and taurine transporter (tau-T) mRNA expression levels as well as cell volume were determined in astrocytes acutely treated with 0.1 and 1.0 mM acrylamide. Statistically significant changes in acrylamide treated astrocytes were noted for GS (0.1 mM) and GLAST (1.0 mM) mRNA expression levels. All other measurements were insignificant in comparison with controls, suggesting that astrocytic function is minimally compromised even at exceedingly high levels of acute acrylamide exposure.

AB - We assessed biochemical endpoints indicative of acute toxicity in neonatal rat primary astrocyte cultures exposed to acrylamide. Metallothionein (MT), glutamine synthetase (GS), glutamate/aspartate transporter (GLAST), and taurine transporter (tau-T) mRNA expression levels as well as cell volume were determined in astrocytes acutely treated with 0.1 and 1.0 mM acrylamide. Statistically significant changes in acrylamide treated astrocytes were noted for GS (0.1 mM) and GLAST (1.0 mM) mRNA expression levels. All other measurements were insignificant in comparison with controls, suggesting that astrocytic function is minimally compromised even at exceedingly high levels of acute acrylamide exposure.

KW - Acrylamide

KW - Astrocyte function

KW - Glutamate

KW - Metallothionein

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JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

The acute effects of acrylamide on astrocyte functions

Abstract

Keywords

ASJC Scopus subject areas

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