The actin-binding protein Lasp promotes Oskar accumulation at the posterior pole of the Drosophila embryo

Ritsuko Suyama, Andreas Jenny, Silvia Curado, Wendy Pellis-van Berkel, Anne Ephrussi

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

During Drosophila oogenesis, oskar mRNA is transported to the posterior pole of the oocyte, where it is locally translated and induces germ-plasm assembly. Oskar protein recruits all of the components necessary for the establishment of posterior embryonic structures and of the germline. Tight localization of Oskar is essential, as its ectopic expression causes severe patterning defects. Here, we show that the Drosophila homolog of mammalian Lasp1 protein, an actin-binding protein previously implicated in cell migration in vertebrate cell culture, contributes to the accumulation of Oskar protein at the posterior pole of the embryo. The reduced number of primordial germ cells in embryos derived from lasp mutant females can be rescued only with a form of Lasp that is capable of interacting with Oskar, revealing the physiological importance of the Lasp-Oskar interaction.

Original languageEnglish (US)
Pages (from-to)95-105
Number of pages11
JournalDevelopment
Volume136
Issue number1
DOIs
StatePublished - Apr 14 2009

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Keywords

  • Actin cytoskeleton
  • Drosophila
  • Lasp
  • Oskar

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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