The ability of multimerized cyclophilin A to restrict retrovirus infection

Hassan Javanbakht, Felipe Diaz-Griffero, Wen Yuan, Darwin F. Yeung, Xing Li, Byeongwoon Song, Joseph Sodroski

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In owl monkeys, the typical retroviral restriction factor of primates, TRIM5α, is replaced by TRIMCyp. TRIMCyp consists of the TRIM5 RING, B-box 2 and coiled-coil domains, as well as the intervening linker regions, fused with cyclophilin A. TRIMCyp restricts infection of retroviruses, such as human immunodeficiency virus (HIV-1) and feline immunodeficiency virus (FIV), with capsids that can bind cyclophilin A. The TRIM5 coiled coil promotes the trimerization of TRIMCyp. Here we show that cyclophilin A that is oligomeric as a result of fusion with a heterologous multimer exhibits substantial antiretroviral activity. The addition of the TRIM5 RING, B-box 2 and Linker 2 to oligomeric cyclophilin A generated a protein with antiretroviral activity approaching that of wild-type TRIMCyp. Multimerization increased the binding of cyclophilin A to the HIV-1 capsid, promoting accelerated uncoating of the capsid and restriction of infection.

Original languageEnglish (US)
Pages (from-to)19-29
Number of pages11
JournalVirology
Volume367
Issue number1
DOIs
StatePublished - Oct 10 2007
Externally publishedYes

Fingerprint

Retroviridae Infections
Cyclophilin A
Capsid
HIV-1
Aotidae
Feline Immunodeficiency Virus
Primates
Infection
Proteins

Keywords

  • Coiled-coil domain
  • Cyclophilin A
  • FIV
  • HIV-1
  • Multimerization
  • Restriction
  • Retroviral capsid
  • TRIM5
  • TRIMCyp

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Javanbakht, H., Diaz-Griffero, F., Yuan, W., Yeung, D. F., Li, X., Song, B., & Sodroski, J. (2007). The ability of multimerized cyclophilin A to restrict retrovirus infection. Virology, 367(1), 19-29. https://doi.org/10.1016/j.virol.2007.04.034

The ability of multimerized cyclophilin A to restrict retrovirus infection. / Javanbakht, Hassan; Diaz-Griffero, Felipe; Yuan, Wen; Yeung, Darwin F.; Li, Xing; Song, Byeongwoon; Sodroski, Joseph.

In: Virology, Vol. 367, No. 1, 10.10.2007, p. 19-29.

Research output: Contribution to journalArticle

Javanbakht, H, Diaz-Griffero, F, Yuan, W, Yeung, DF, Li, X, Song, B & Sodroski, J 2007, 'The ability of multimerized cyclophilin A to restrict retrovirus infection', Virology, vol. 367, no. 1, pp. 19-29. https://doi.org/10.1016/j.virol.2007.04.034
Javanbakht, Hassan ; Diaz-Griffero, Felipe ; Yuan, Wen ; Yeung, Darwin F. ; Li, Xing ; Song, Byeongwoon ; Sodroski, Joseph. / The ability of multimerized cyclophilin A to restrict retrovirus infection. In: Virology. 2007 ; Vol. 367, No. 1. pp. 19-29.
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