The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans

M. Fan, L. C. Chen, M. A. Ragan, R. R. Gutell, J. R. Warner, B. P. Currie, A. Casadevall

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The intergenic spacers (IGS) separating the 23S-like and 16S-like rDNAs of the two varieties of the human pathogenic fungus Cryptococcus neoformans were amplified, cloned and sequenced. The C. neoformans var. neoformans IGS was 2421 nt with 5S rRNA at positions 1228-1345 3' of the 23S-like rRNA. The C. neoformans var. gattii IGS was 2480 nt with 5S rRNA at positions 1268-1385 3' of the 23S-like rRNA. For both varieties the 5S rDNA genes were in the same orientation as the 16S-5.8-23S genes and encode a 118 nt molecule of identical sequence. Phylogenetic comparison of C. neoformans 5S rDNA with that of other fungi placed this fungus in close relationship with other basidiomycetes including Tremella mesenterica, Bullera alba, and Cryptococcus laurentii. A secondary structure model for the deduced 5S rRNA was constructed by comparative sequence analysis. Polymerase chain reaction-amplified IGS of 12 C. neoformans var. neoformans strains revealed extensive size variation ranging from 100 to 300 nt. Size variation between strains in the length of the IGS may be useful for distinguishing strains. Structurally, the IGS were characterized by the presence of occasional short direct GC-rich 19-nt repeats. Overall IGS sequence identity between the C. neoformans varieties was only 78.5%, in sharp contrast to the identical or nearly identical sequences for the rDNA genes, and suggests rapid evolution for IGS sequences.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalJournal of Medical and Veterinary Mycology
Volume33
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Cryptococcus neoformans
Ribosomal DNA
Intergenic DNA
Fungi
Genes
Cryptococcus
Basidiomycota
Sequence Analysis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology

Cite this

Fan, M., Chen, L. C., Ragan, M. A., Gutell, R. R., Warner, J. R., Currie, B. P., & Casadevall, A. (1995). The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans. Journal of Medical and Veterinary Mycology, 33(4), 215-221.

The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans. / Fan, M.; Chen, L. C.; Ragan, M. A.; Gutell, R. R.; Warner, J. R.; Currie, B. P.; Casadevall, A.

In: Journal of Medical and Veterinary Mycology, Vol. 33, No. 4, 1995, p. 215-221.

Research output: Contribution to journalArticle

Fan, M, Chen, LC, Ragan, MA, Gutell, RR, Warner, JR, Currie, BP & Casadevall, A 1995, 'The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans', Journal of Medical and Veterinary Mycology, vol. 33, no. 4, pp. 215-221.
Fan M, Chen LC, Ragan MA, Gutell RR, Warner JR, Currie BP et al. The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans. Journal of Medical and Veterinary Mycology. 1995;33(4):215-221.
Fan, M. ; Chen, L. C. ; Ragan, M. A. ; Gutell, R. R. ; Warner, J. R. ; Currie, B. P. ; Casadevall, A. / The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans. In: Journal of Medical and Veterinary Mycology. 1995 ; Vol. 33, No. 4. pp. 215-221.
@article{c7ceb7a5ca7b4172bcd2d6b2127ffb9a,
title = "The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans",
abstract = "The intergenic spacers (IGS) separating the 23S-like and 16S-like rDNAs of the two varieties of the human pathogenic fungus Cryptococcus neoformans were amplified, cloned and sequenced. The C. neoformans var. neoformans IGS was 2421 nt with 5S rRNA at positions 1228-1345 3' of the 23S-like rRNA. The C. neoformans var. gattii IGS was 2480 nt with 5S rRNA at positions 1268-1385 3' of the 23S-like rRNA. For both varieties the 5S rDNA genes were in the same orientation as the 16S-5.8-23S genes and encode a 118 nt molecule of identical sequence. Phylogenetic comparison of C. neoformans 5S rDNA with that of other fungi placed this fungus in close relationship with other basidiomycetes including Tremella mesenterica, Bullera alba, and Cryptococcus laurentii. A secondary structure model for the deduced 5S rRNA was constructed by comparative sequence analysis. Polymerase chain reaction-amplified IGS of 12 C. neoformans var. neoformans strains revealed extensive size variation ranging from 100 to 300 nt. Size variation between strains in the length of the IGS may be useful for distinguishing strains. Structurally, the IGS were characterized by the presence of occasional short direct GC-rich 19-nt repeats. Overall IGS sequence identity between the C. neoformans varieties was only 78.5{\%}, in sharp contrast to the identical or nearly identical sequences for the rDNA genes, and suggests rapid evolution for IGS sequences.",
author = "M. Fan and Chen, {L. C.} and Ragan, {M. A.} and Gutell, {R. R.} and Warner, {J. R.} and Currie, {B. P.} and A. Casadevall",
year = "1995",
language = "English (US)",
volume = "33",
pages = "215--221",
journal = "Medical Mycology",
issn = "1369-3786",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - The 5S rRNA and the rRNA intergenic spacer of the two varieties of Cryptococcus neoformans

AU - Fan, M.

AU - Chen, L. C.

AU - Ragan, M. A.

AU - Gutell, R. R.

AU - Warner, J. R.

AU - Currie, B. P.

AU - Casadevall, A.

PY - 1995

Y1 - 1995

N2 - The intergenic spacers (IGS) separating the 23S-like and 16S-like rDNAs of the two varieties of the human pathogenic fungus Cryptococcus neoformans were amplified, cloned and sequenced. The C. neoformans var. neoformans IGS was 2421 nt with 5S rRNA at positions 1228-1345 3' of the 23S-like rRNA. The C. neoformans var. gattii IGS was 2480 nt with 5S rRNA at positions 1268-1385 3' of the 23S-like rRNA. For both varieties the 5S rDNA genes were in the same orientation as the 16S-5.8-23S genes and encode a 118 nt molecule of identical sequence. Phylogenetic comparison of C. neoformans 5S rDNA with that of other fungi placed this fungus in close relationship with other basidiomycetes including Tremella mesenterica, Bullera alba, and Cryptococcus laurentii. A secondary structure model for the deduced 5S rRNA was constructed by comparative sequence analysis. Polymerase chain reaction-amplified IGS of 12 C. neoformans var. neoformans strains revealed extensive size variation ranging from 100 to 300 nt. Size variation between strains in the length of the IGS may be useful for distinguishing strains. Structurally, the IGS were characterized by the presence of occasional short direct GC-rich 19-nt repeats. Overall IGS sequence identity between the C. neoformans varieties was only 78.5%, in sharp contrast to the identical or nearly identical sequences for the rDNA genes, and suggests rapid evolution for IGS sequences.

AB - The intergenic spacers (IGS) separating the 23S-like and 16S-like rDNAs of the two varieties of the human pathogenic fungus Cryptococcus neoformans were amplified, cloned and sequenced. The C. neoformans var. neoformans IGS was 2421 nt with 5S rRNA at positions 1228-1345 3' of the 23S-like rRNA. The C. neoformans var. gattii IGS was 2480 nt with 5S rRNA at positions 1268-1385 3' of the 23S-like rRNA. For both varieties the 5S rDNA genes were in the same orientation as the 16S-5.8-23S genes and encode a 118 nt molecule of identical sequence. Phylogenetic comparison of C. neoformans 5S rDNA with that of other fungi placed this fungus in close relationship with other basidiomycetes including Tremella mesenterica, Bullera alba, and Cryptococcus laurentii. A secondary structure model for the deduced 5S rRNA was constructed by comparative sequence analysis. Polymerase chain reaction-amplified IGS of 12 C. neoformans var. neoformans strains revealed extensive size variation ranging from 100 to 300 nt. Size variation between strains in the length of the IGS may be useful for distinguishing strains. Structurally, the IGS were characterized by the presence of occasional short direct GC-rich 19-nt repeats. Overall IGS sequence identity between the C. neoformans varieties was only 78.5%, in sharp contrast to the identical or nearly identical sequences for the rDNA genes, and suggests rapid evolution for IGS sequences.

UR - http://www.scopus.com/inward/record.url?scp=0029149196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029149196&partnerID=8YFLogxK

M3 - Article

C2 - 8531018

AN - SCOPUS:0029149196

VL - 33

SP - 215

EP - 221

JO - Medical Mycology

JF - Medical Mycology

SN - 1369-3786

IS - 4

ER -