TY - JOUR
T1 - The 29 kDa light chain that regulates axonemal dynein activity binds to cytoplasmic dyneins
AU - Wang, Hali
AU - Satir, Peter
PY - 1998
Y1 - 1998
N2 - In earlier studies, Hamasaki et al. (Proc. Natl. Acad. USA. 88:7918- 7922, 1991) and Barkalow et al. (J. Cell Biol. 126:727-735, 1994) found that cAMP- and Ca2+-sensitive phosphorylation of a 29 kDa dynein light chain (p29) extracted from 22S axonemal dynein of Paramecium, regulates the velocity of in vitro microtubule translocation and ciliate swimming speed. In this study we report evidence of recombination of p29 to cytoplasmic dyneins from both rat liver and Paramecium, as well as to 22S dynein precursor molecule, based on immunoprecipitation and force filtration data. Immunoprecipitation also provides additional evidence for the binding of p29 to 22S axonemal dynein. The results suggest that p29 might regulate cytoplasmic dynein, as well as axonemal dynein function in Paramecium, and that a homologue of 29 may exist in rat liver and other mammalian cells.
AB - In earlier studies, Hamasaki et al. (Proc. Natl. Acad. USA. 88:7918- 7922, 1991) and Barkalow et al. (J. Cell Biol. 126:727-735, 1994) found that cAMP- and Ca2+-sensitive phosphorylation of a 29 kDa dynein light chain (p29) extracted from 22S axonemal dynein of Paramecium, regulates the velocity of in vitro microtubule translocation and ciliate swimming speed. In this study we report evidence of recombination of p29 to cytoplasmic dyneins from both rat liver and Paramecium, as well as to 22S dynein precursor molecule, based on immunoprecipitation and force filtration data. Immunoprecipitation also provides additional evidence for the binding of p29 to 22S axonemal dynein. The results suggest that p29 might regulate cytoplasmic dynein, as well as axonemal dynein function in Paramecium, and that a homologue of 29 may exist in rat liver and other mammalian cells.
KW - Axoneme
KW - Cytoplasmic dynein
KW - Paramecium
KW - Rat liter
KW - Regulatory light chain
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U2 - 10.1002/(SICI)1097-0169(1998)39:1<1::AID-CM1>3.0.CO;2-B
DO - 10.1002/(SICI)1097-0169(1998)39:1<1::AID-CM1>3.0.CO;2-B
M3 - Article
C2 - 9453709
AN - SCOPUS:0031972777
SN - 0886-1544
VL - 39
SP - 1
EP - 8
JO - Cell Motility and the Cytoskeleton
JF - Cell Motility and the Cytoskeleton
IS - 1
ER -