The 1.8 Å crystal structure of catechol 1,2-dioxygenase reveals a novel hydrophobic helical zipper as a subunit linker

Matthew W. Vetting, Douglas H. Ohlendorf

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

Background: Intradiol dioxygenases catalyze the critical ring-cleavage step in the conversion of catecholate derivatives to citric acid cycle intermediates. Catechol 1,2-dioxygenases (1,2-CTDs) have a rudimentary design structure - a homodimer with one catalytic non-berne ferric ion per monomer, that is (αFe3+)2. This is in contrast to the archetypical intradiol dioxygenase protocatechuate 3,4-dioxygenase (3,4-PCD), which forms more diverse oligomers, such as (αFe3+)2-12. Results: The crystal structure of 1,2-CTD from Acinetobacter sp. ADP1 (Ac 1,2-CTD) was solved by single isomorphous replacement and refined to 2.0 Å resolution. The structures of the enzyme complexed with catechol and 4-methylcatechol were also determined at resolutions of 1.9 Å and 1.8 Å, respectively. While the characteristics of the iron ligands are similar, Ac 1,2-CTD differs from 3,4-PCDs in that only one subunit is used to fashion each active-site cavity. In addition, a novel 'helical zipper', consisting of five N-terminal helices from each subunit, forms the molecular dimer axis. Two phospholipids were unexpectedly found to bind within an 8 x 35 Å hydrophobic tunnel along this axis. Conclusions: The helical zipper domain of Ac 1,2-CTD has no equivalent in other proteins of known structure. Sequence analysis suggests the domain is a common motif in all members of the 1,2-CTD family. Complexes with catechol and 4-methylcatechol are the highest resolution complex structures to date of an intradiol dioxygenase. Furthermore, they confirm several observations seen in 3,4-PCDs, including ligand displacement upon binding exogenous ligands. The structures presented here are the first of a new family of intradiol dioxygenases.

Original languageEnglish (US)
Pages (from-to)429-440
Number of pages12
JournalStructure
Volume8
Issue number4
DOIs
StatePublished - Apr 1 2000

Keywords

  • Aromatic catabolism
  • Intradiol dioxygenase
  • Ligand dissociation
  • Metalloenzyme
  • Phospholipid

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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