The β-major and β-minor globin genes in murine erythroleukemia cells replicate during the same early interval of the S phase

J. D. Braunstein, C. L. Schildkraut

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Abstract

The time of replication in S phase was determined for the 7.3 kb EcoRI segment containing the β-major globin gene and the 14 kb EcoRI segment containing the β-minor globin gene in a murine Friend erythroleukemia virus transformed (MEL) cell line. Cells were obtained from different intervals of S phase by centrifugal elutriation to avoid artifacts of chemical synchronization. Newly synthesized DNA from different parts of S phase were obtained by isolation of 5-bromouracil (BU) labelled DNA from these cells. The BU-DNA synthesized during four different intervals of S was transferred to diazobenzyloxymethyl (DBM) paper and hybridized with a β-globin cDNA probe. Quantitation showed that both β-globin segments were replicated in the first quarter of the S phase with no significant difference in their time of replication in this MEL cell line.

Original languageEnglish (US)
Pages (from-to)108-113
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume123
Issue number1
DOIs
Publication statusPublished - Aug 30 1984

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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