The β-major and β-minor globin genes in murine erythroleukemia cells replicate during the same early interval of the S phase

J. D. Braunstein, Carl L. Schildkraut

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The time of replication in S phase was determined for the 7.3 kb EcoRI segment containing the β-major globin gene and the 14 kb EcoRI segment containing the β-minor globin gene in a murine Friend erythroleukemia virus transformed (MEL) cell line. Cells were obtained from different intervals of S phase by centrifugal elutriation to avoid artifacts of chemical synchronization. Newly synthesized DNA from different parts of S phase were obtained by isolation of 5-bromouracil (BU) labelled DNA from these cells. The BU-DNA synthesized during four different intervals of S was transferred to diazobenzyloxymethyl (DBM) paper and hybridized with a β-globin cDNA probe. Quantitation showed that both β-globin segments were replicated in the first quarter of the S phase with no significant difference in their time of replication in this MEL cell line.

Original languageEnglish (US)
Pages (from-to)108-113
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume123
Issue number1
DOIs
StatePublished - Aug 30 1984

Fingerprint

Leukemia, Erythroblastic, Acute
Globins
S Phase
Bromouracil
Genes
DNA
Cells
Friend murine leukemia virus
Transformed Cell Line
Viruses
Artifacts
Synchronization
Complementary DNA
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

@article{71894eb7efb7468c9bd096b64cba1e62,
title = "The β-major and β-minor globin genes in murine erythroleukemia cells replicate during the same early interval of the S phase",
abstract = "The time of replication in S phase was determined for the 7.3 kb EcoRI segment containing the β-major globin gene and the 14 kb EcoRI segment containing the β-minor globin gene in a murine Friend erythroleukemia virus transformed (MEL) cell line. Cells were obtained from different intervals of S phase by centrifugal elutriation to avoid artifacts of chemical synchronization. Newly synthesized DNA from different parts of S phase were obtained by isolation of 5-bromouracil (BU) labelled DNA from these cells. The BU-DNA synthesized during four different intervals of S was transferred to diazobenzyloxymethyl (DBM) paper and hybridized with a β-globin cDNA probe. Quantitation showed that both β-globin segments were replicated in the first quarter of the S phase with no significant difference in their time of replication in this MEL cell line.",
author = "Braunstein, {J. D.} and Schildkraut, {Carl L.}",
year = "1984",
month = "8",
day = "30",
doi = "10.1016/0006-291X(84)90386-3",
language = "English (US)",
volume = "123",
pages = "108--113",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The β-major and β-minor globin genes in murine erythroleukemia cells replicate during the same early interval of the S phase

AU - Braunstein, J. D.

AU - Schildkraut, Carl L.

PY - 1984/8/30

Y1 - 1984/8/30

N2 - The time of replication in S phase was determined for the 7.3 kb EcoRI segment containing the β-major globin gene and the 14 kb EcoRI segment containing the β-minor globin gene in a murine Friend erythroleukemia virus transformed (MEL) cell line. Cells were obtained from different intervals of S phase by centrifugal elutriation to avoid artifacts of chemical synchronization. Newly synthesized DNA from different parts of S phase were obtained by isolation of 5-bromouracil (BU) labelled DNA from these cells. The BU-DNA synthesized during four different intervals of S was transferred to diazobenzyloxymethyl (DBM) paper and hybridized with a β-globin cDNA probe. Quantitation showed that both β-globin segments were replicated in the first quarter of the S phase with no significant difference in their time of replication in this MEL cell line.

AB - The time of replication in S phase was determined for the 7.3 kb EcoRI segment containing the β-major globin gene and the 14 kb EcoRI segment containing the β-minor globin gene in a murine Friend erythroleukemia virus transformed (MEL) cell line. Cells were obtained from different intervals of S phase by centrifugal elutriation to avoid artifacts of chemical synchronization. Newly synthesized DNA from different parts of S phase were obtained by isolation of 5-bromouracil (BU) labelled DNA from these cells. The BU-DNA synthesized during four different intervals of S was transferred to diazobenzyloxymethyl (DBM) paper and hybridized with a β-globin cDNA probe. Quantitation showed that both β-globin segments were replicated in the first quarter of the S phase with no significant difference in their time of replication in this MEL cell line.

UR - http://www.scopus.com/inward/record.url?scp=0021171853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021171853&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(84)90386-3

DO - 10.1016/0006-291X(84)90386-3

M3 - Article

C2 - 6477576

AN - SCOPUS:0021171853

VL - 123

SP - 108

EP - 113

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -