Abstract
The time of replication in S phase was determined for the 7.3 kb EcoRI segment containing the β-major globin gene and the 14 kb EcoRI segment containing the β-minor globin gene in a murine Friend erythroleukemia virus transformed (MEL) cell line. Cells were obtained from different intervals of S phase by centrifugal elutriation to avoid artifacts of chemical synchronization. Newly synthesized DNA from different parts of S phase were obtained by isolation of 5-bromouracil (BU) labelled DNA from these cells. The BU-DNA synthesized during four different intervals of S was transferred to diazobenzyloxymethyl (DBM) paper and hybridized with a β-globin cDNA probe. Quantitation showed that both β-globin segments were replicated in the first quarter of the S phase with no significant difference in their time of replication in this MEL cell line.
Original language | English (US) |
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Pages (from-to) | 108-113 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 123 |
Issue number | 1 |
DOIs | |
State | Published - Aug 30 1984 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology