TY - JOUR
T1 - The β subunit of the guanine nucleotide regulatory proteins
T2 - Identification of its location on two‐dimesional gels of brain tissue and its regional and subcellular distribution in brain
AU - Heydorn, W. E.
AU - Gierschik, P.
AU - Creed, G. J.
AU - Milligan, G.
AU - Spiegel, A.
AU - Jacobowitz, D. M.
PY - 1986
Y1 - 1986
N2 - The β subunit of the guanine nucleotide regulatory proteins (also termed G proteins) has been examined in both rat and human brain, a single protein of molecular weight 36,000 daltons and pI 5.8 reacted the antisera. This protein also comigrated with one of the proteins present in a purified preparation of bovine brain G proteins. Based upon molecular weight, pI, and reactiion with specific antisera, it was concluded that this protein is the β subunit of the G proteins in brain. Using this information, the regional and subcellular distribution of the G protein β subunit was studied in rat brain. Of 25 distinct neuroanatomical areas examined, cortical regions were generally found to contain the largest amount of this protein. The subcellular distribution of the G protein β subunit revealed that large amounts are present in the synaptic membrane, crude synaptic vesicles, and microsomes. These studies serve to identify another protein visible on silver‐stained two‐dimensional electrophortograms of rat and human brain. The regional and subcellular distribution of the G protein β subunit correlate well with the proposed physiological function of this protein.
AB - The β subunit of the guanine nucleotide regulatory proteins (also termed G proteins) has been examined in both rat and human brain, a single protein of molecular weight 36,000 daltons and pI 5.8 reacted the antisera. This protein also comigrated with one of the proteins present in a purified preparation of bovine brain G proteins. Based upon molecular weight, pI, and reactiion with specific antisera, it was concluded that this protein is the β subunit of the G proteins in brain. Using this information, the regional and subcellular distribution of the G protein β subunit was studied in rat brain. Of 25 distinct neuroanatomical areas examined, cortical regions were generally found to contain the largest amount of this protein. The subcellular distribution of the G protein β subunit revealed that large amounts are present in the synaptic membrane, crude synaptic vesicles, and microsomes. These studies serve to identify another protein visible on silver‐stained two‐dimensional electrophortograms of rat and human brain. The regional and subcellular distribution of the G protein β subunit correlate well with the proposed physiological function of this protein.
KW - G protein β subunit
KW - protein blotting
KW - two‐dimensional gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0023023387&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023023387&partnerID=8YFLogxK
U2 - 10.1002/jnr.490160309
DO - 10.1002/jnr.490160309
M3 - Article
C2 - 2430105
AN - SCOPUS:0023023387
SN - 0360-4012
VL - 16
SP - 541
EP - 552
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 3
ER -