Testosterone treatment of human foreskin in a novel transplant model

Joshua M. Stern, Jie Chen, Sara B. Peters, Peter J. Stahl, Maher El-Chaar, Diane Felsen, Dix P. Poppas

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objectives To determine whether testosterone would increase angiogenesis in human foreskin, because of the known effects of testosterone on endothelial cells and vascular endothelial growth factor expression. Surgical management of complex hypospadias in a patient who has undergone multiple procedures is technically very challenging. It is possible that therapy to increase the blood supply to this tissue could be beneficial. Methods We used a newly developed model of human skin transplantation, in which full-thickness human foreskin is transplanted subcutaneously onto the dorsum of a nude rat. At 10 days after transplantation, tissue was treated with either testosterone gel or vehicle control. After an additional 7 days, the tissue was harvested, embedded in paraffin, stained for factor VIII to assess vascularity, and examined histologically. Results The testosterone-treated tissue demonstrated increased factor VIII staining (31.14 ± 1.53 vessels per high-power field) compared with the control group (18.25 ± 2.3 vessels per high-power field; P <0.0005). Histologic analysis revealed less collagen in the testosterone-treated group compared with the control group. Conclusions Treatment of human foreskin with testosterone, in a transplant model, increased vascularity and decreased early fibrosis. Testosterone treatment may improve the surgical management of complex hypospadias repair.

Original languageEnglish (US)
Pages (from-to)999-1003
Number of pages5
JournalUrology
Volume63
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Foreskin
Testosterone
Transplants
Hypospadias
Factor VIII
Therapeutics
Nude Rats
Tissue Transplantation
Control Groups
Skin Transplantation
Paraffin
Vascular Endothelial Growth Factor A
Fibrosis
Collagen
Endothelial Cells
Gels
Staining and Labeling

ASJC Scopus subject areas

  • Urology

Cite this

Stern, J. M., Chen, J., Peters, S. B., Stahl, P. J., El-Chaar, M., Felsen, D., & Poppas, D. P. (2004). Testosterone treatment of human foreskin in a novel transplant model. Urology, 63(5), 999-1003. https://doi.org/10.1016/j.urology.2003.11.033

Testosterone treatment of human foreskin in a novel transplant model. / Stern, Joshua M.; Chen, Jie; Peters, Sara B.; Stahl, Peter J.; El-Chaar, Maher; Felsen, Diane; Poppas, Dix P.

In: Urology, Vol. 63, No. 5, 05.2004, p. 999-1003.

Research output: Contribution to journalArticle

Stern, JM, Chen, J, Peters, SB, Stahl, PJ, El-Chaar, M, Felsen, D & Poppas, DP 2004, 'Testosterone treatment of human foreskin in a novel transplant model', Urology, vol. 63, no. 5, pp. 999-1003. https://doi.org/10.1016/j.urology.2003.11.033
Stern JM, Chen J, Peters SB, Stahl PJ, El-Chaar M, Felsen D et al. Testosterone treatment of human foreskin in a novel transplant model. Urology. 2004 May;63(5):999-1003. https://doi.org/10.1016/j.urology.2003.11.033
Stern, Joshua M. ; Chen, Jie ; Peters, Sara B. ; Stahl, Peter J. ; El-Chaar, Maher ; Felsen, Diane ; Poppas, Dix P. / Testosterone treatment of human foreskin in a novel transplant model. In: Urology. 2004 ; Vol. 63, No. 5. pp. 999-1003.
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