Testosterone regulates erectile function and Vcsa1 expression in the corpora of rats

Rowena G. Chua, Giulia Calenda, Xinhua Zhang, Joseph Siragusa, Yuehong Tong, Moses T. Tar, Memduh Aydin, Michael E. DiSanto, Arnold Melman, Kelvin Davies

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Vcsa1 plays an important role in the erectile physiology of the rat. We conducted experiments to determine if erectile function, testosterone levels and Vcsa1 expression were correlated. In orchiectomized rats, total testosterone in blood fell from an average of 4 ng/ml to <0.04 ng/ml. Erectile function was significantly lower compared to controls and Vcsa1 expression was significantly (>6-fold) decreased. Injection of orchiectomized animals with testosterone (2 mg in 100 ml sesame oil every 4 days for 2 weeks) restored average levels of testosterone to 2 ng/ml, increased erectile function and significantly increased Vcsa1 expression. In isolated corporal cells there was testosterone dependent Vcsa1 expression. However, intracorporal injection of orchiectomized animals with a plasmid expressing Vcsa1 or its gene product Sialorphin (previously demonstrated to improve erectile function in old animals) gave no significant improvement in erectile function. Also, the ability of Sialorphin to reduce tension in corporal smooth muscle strips isolated from orchiectomized animals was impaired compared to controls.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume303
Issue number1-2
DOIs
StatePublished - May 6 2009

Fingerprint

Testosterone
Rats
Animals
Sesame Oil
Injections
Physiology
Smooth Muscle
Muscle
Plasmids
Blood
Genes
Experiments

Keywords

  • Corpora
  • Erectile function
  • Submandibular gland
  • Testosterone
  • Vcsa1

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Testosterone regulates erectile function and Vcsa1 expression in the corpora of rats. / Chua, Rowena G.; Calenda, Giulia; Zhang, Xinhua; Siragusa, Joseph; Tong, Yuehong; Tar, Moses T.; Aydin, Memduh; DiSanto, Michael E.; Melman, Arnold; Davies, Kelvin.

In: Molecular and Cellular Endocrinology, Vol. 303, No. 1-2, 06.05.2009, p. 67-73.

Research output: Contribution to journalArticle

Chua, RG, Calenda, G, Zhang, X, Siragusa, J, Tong, Y, Tar, MT, Aydin, M, DiSanto, ME, Melman, A & Davies, K 2009, 'Testosterone regulates erectile function and Vcsa1 expression in the corpora of rats', Molecular and Cellular Endocrinology, vol. 303, no. 1-2, pp. 67-73. https://doi.org/10.1016/j.mce.2009.02.001
Chua, Rowena G. ; Calenda, Giulia ; Zhang, Xinhua ; Siragusa, Joseph ; Tong, Yuehong ; Tar, Moses T. ; Aydin, Memduh ; DiSanto, Michael E. ; Melman, Arnold ; Davies, Kelvin. / Testosterone regulates erectile function and Vcsa1 expression in the corpora of rats. In: Molecular and Cellular Endocrinology. 2009 ; Vol. 303, No. 1-2. pp. 67-73.
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