Testosterone induces expression of transforming growth factor-beta1 in the murine thymus

Nancy J. Olsen, Ping Zhou, Helena Ong, William J. Kovacs

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Castration of adult male mice results in enlargement of the thymus and diminution of peripheral suppressor T cell function. Replacement therapy with physiologic doses of androgens reverses these phenomena. Although the mediators involved are unknown, these effects of androgens on the thymus and peripheral immune system are reminiscent of those reported for transforming growth factor-beta (TGF-β1). We examined expression of TGF-β1 mRNA and bioactive protein in thymuses from castrate and androgen-replaced animals. Steady-state levels of thymic TGF-β1 mRNA fell slightly after castration, but rose 2.3-fold after testosterone replacement. Bioactive TGF-β1 production by cultured thymic explants also fell following castration to approx. 50% of the levels observed in intact animals. Following 1 week of testosterone replacement in castrate animals, TGF-β1 bioactivity produced in culture was restored to levels indistinguishable from those observed with explants from intact animals. Reverse transcription/polymerase chain reaction amplification of RNA revealed that thymocytes are a source of the androgen-modulated TGF-β1. These results suggest that TGF-β1 may mediate effects of androgens on the immune system.

Original languageEnglish (US)
Pages (from-to)327-332
Number of pages6
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume45
Issue number5
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Transforming Growth Factor beta1
Thymus
Thymus Gland
Androgens
Testosterone
Animals
Immune system
Castration
Immune System
Messenger RNA
T-cells
Orchiectomy
Polymerase chain reaction
Thymocytes
Transcription
Bioactivity
Transforming Growth Factor beta
Reverse Transcription
Amplification
RNA

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Testosterone induces expression of transforming growth factor-beta1 in the murine thymus. / Olsen, Nancy J.; Zhou, Ping; Ong, Helena; Kovacs, William J.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 45, No. 5, 1993, p. 327-332.

Research output: Contribution to journalArticle

@article{62d1ef15ba2c4ad7a1b921c6d62e7f40,
title = "Testosterone induces expression of transforming growth factor-beta1 in the murine thymus",
abstract = "Castration of adult male mice results in enlargement of the thymus and diminution of peripheral suppressor T cell function. Replacement therapy with physiologic doses of androgens reverses these phenomena. Although the mediators involved are unknown, these effects of androgens on the thymus and peripheral immune system are reminiscent of those reported for transforming growth factor-beta (TGF-β1). We examined expression of TGF-β1 mRNA and bioactive protein in thymuses from castrate and androgen-replaced animals. Steady-state levels of thymic TGF-β1 mRNA fell slightly after castration, but rose 2.3-fold after testosterone replacement. Bioactive TGF-β1 production by cultured thymic explants also fell following castration to approx. 50{\%} of the levels observed in intact animals. Following 1 week of testosterone replacement in castrate animals, TGF-β1 bioactivity produced in culture was restored to levels indistinguishable from those observed with explants from intact animals. Reverse transcription/polymerase chain reaction amplification of RNA revealed that thymocytes are a source of the androgen-modulated TGF-β1. These results suggest that TGF-β1 may mediate effects of androgens on the immune system.",
author = "Olsen, {Nancy J.} and Ping Zhou and Helena Ong and Kovacs, {William J.}",
year = "1993",
doi = "10.1016/0960-0760(93)90001-D",
language = "English (US)",
volume = "45",
pages = "327--332",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
issn = "0960-0760",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Testosterone induces expression of transforming growth factor-beta1 in the murine thymus

AU - Olsen, Nancy J.

AU - Zhou, Ping

AU - Ong, Helena

AU - Kovacs, William J.

PY - 1993

Y1 - 1993

N2 - Castration of adult male mice results in enlargement of the thymus and diminution of peripheral suppressor T cell function. Replacement therapy with physiologic doses of androgens reverses these phenomena. Although the mediators involved are unknown, these effects of androgens on the thymus and peripheral immune system are reminiscent of those reported for transforming growth factor-beta (TGF-β1). We examined expression of TGF-β1 mRNA and bioactive protein in thymuses from castrate and androgen-replaced animals. Steady-state levels of thymic TGF-β1 mRNA fell slightly after castration, but rose 2.3-fold after testosterone replacement. Bioactive TGF-β1 production by cultured thymic explants also fell following castration to approx. 50% of the levels observed in intact animals. Following 1 week of testosterone replacement in castrate animals, TGF-β1 bioactivity produced in culture was restored to levels indistinguishable from those observed with explants from intact animals. Reverse transcription/polymerase chain reaction amplification of RNA revealed that thymocytes are a source of the androgen-modulated TGF-β1. These results suggest that TGF-β1 may mediate effects of androgens on the immune system.

AB - Castration of adult male mice results in enlargement of the thymus and diminution of peripheral suppressor T cell function. Replacement therapy with physiologic doses of androgens reverses these phenomena. Although the mediators involved are unknown, these effects of androgens on the thymus and peripheral immune system are reminiscent of those reported for transforming growth factor-beta (TGF-β1). We examined expression of TGF-β1 mRNA and bioactive protein in thymuses from castrate and androgen-replaced animals. Steady-state levels of thymic TGF-β1 mRNA fell slightly after castration, but rose 2.3-fold after testosterone replacement. Bioactive TGF-β1 production by cultured thymic explants also fell following castration to approx. 50% of the levels observed in intact animals. Following 1 week of testosterone replacement in castrate animals, TGF-β1 bioactivity produced in culture was restored to levels indistinguishable from those observed with explants from intact animals. Reverse transcription/polymerase chain reaction amplification of RNA revealed that thymocytes are a source of the androgen-modulated TGF-β1. These results suggest that TGF-β1 may mediate effects of androgens on the immune system.

UR - http://www.scopus.com/inward/record.url?scp=0027279048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027279048&partnerID=8YFLogxK

U2 - 10.1016/0960-0760(93)90001-D

DO - 10.1016/0960-0760(93)90001-D

M3 - Article

VL - 45

SP - 327

EP - 332

JO - Journal of Steroid Biochemistry and Molecular Biology

JF - Journal of Steroid Biochemistry and Molecular Biology

SN - 0960-0760

IS - 5

ER -