Testicular niche required for human spermatogonial stem cell expansion

James F. Smith, Pamela Yango, Eran Altman, Shweta Choudhry, Andrea Poelzl, Alberuni M. Zamah, Mitchell Rosen, Peter C. Klatsky, Nam D. Tran

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Prepubertal boys treated with high-dose chemotherapy do not have an established means of fertility preservation because no established in vitro technique exists to expand and mature purified spermatogonial stem cells (SSCs) to functional sperm in humans. In this study, we define and characterize the unique testicular cellular niche required for SSC expansion using testicular tissues from men with normal spermatogenesis. Highly purified SSCs and testicular somatic cells were isolated by fluorescence-activated cell sorting using SSEA-4 and THY1 as markers of SSCs and somatic cells. Cells were cultured on various established niches to assess their role in SSC expansion in a defined somatic cellular niche. Of all the niches examined, cells in the SSEA-4 population exclusively bound to adult testicular stromal cells, established colonies, and expanded. Further characterization of these testicular stromal cells revealed distinct mesenchymal markers and the ability to undergo differentiation along the mesenchymal lineage, supporting a testicular multipotent stromal cell origin. In vitro human SSC expansion requires a unique niche provided exclusively by testicular multipotent stromal cells with mesenchymal properties. These findings provide an important foundation for developing methods of inducing SSC growth and maturation in prepubertal testicular tissue, essential to enabling fertility preservation for these boys.

Original languageEnglish (US)
Pages (from-to)1043-1054
Number of pages12
JournalStem Cells Translational Medicine
Volume3
Issue number9
DOIs
StatePublished - 2014

    Fingerprint

Keywords

  • Cancer
  • Cell surface markers
  • Clinical translation
  • Mesenchymal stem cells
  • Spermatogonial stem cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Smith, J. F., Yango, P., Altman, E., Choudhry, S., Poelzl, A., Zamah, A. M., Rosen, M., Klatsky, P. C., & Tran, N. D. (2014). Testicular niche required for human spermatogonial stem cell expansion. Stem Cells Translational Medicine, 3(9), 1043-1054. https://doi.org/10.5966/sctm.2014-0045