Terminal minihelix, a novel RNA motif that directs polymerase III transcripts to the cell cytoplasm

Terminal minihelix and RNA export

Carole Gwizdek, Edouard Bertrand, Catherine Dargemont, Jean Claude Lefebvre, Jean Marie Blanchard, Robert H. Singer, Alain Doglio

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Determining the cis-acting elements controlling nuclear export of RNA is critical, because they specify which RNA will be selected for transport. We have characterized the nuclear export motif of the adenoviral VA1 RNA, a small cytoplasmic RNA transcribed by RNA polymerase III. Using a large panel of VA1 mutants in both transfected COS cells and injected Xenopus oocytes, we showed that the terminal stem of VA1 is necessary and sufficient for its export. Surprisingly, we found that the nucleotide sequence within the terminal stem is not important. Rather, the salient features of this motif are its length and its relative position within the RNA. Such stems thus define a novel and degenerate cytoplasmic localization motif that we termed the minihelix. This motif is found in a variety of polymerase III transcripts, and cross-competition analysis in Xenopus oocytes revealed that export of one such RNA, like hY1 RNA, is specifically competed by VA1 or artificial minihelix. Taken together these results show that the minihelix defines a new cis-acting export element and that this motif could be exported via a novel and specific nuclear export pathway.

Original languageEnglish (US)
Pages (from-to)25910-25918
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number28
DOIs
StatePublished - Jul 13 2001

Fingerprint

Nucleotide Motifs
Cytoplasm
RNA
Cell Nucleus Active Transport
Xenopus
Oocytes
Small Cytoplasmic RNA
RNA Polymerase III
Nuclear RNA
COS Cells
Nucleotides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Terminal minihelix, a novel RNA motif that directs polymerase III transcripts to the cell cytoplasm : Terminal minihelix and RNA export. / Gwizdek, Carole; Bertrand, Edouard; Dargemont, Catherine; Lefebvre, Jean Claude; Blanchard, Jean Marie; Singer, Robert H.; Doglio, Alain.

In: Journal of Biological Chemistry, Vol. 276, No. 28, 13.07.2001, p. 25910-25918.

Research output: Contribution to journalArticle

Gwizdek, Carole ; Bertrand, Edouard ; Dargemont, Catherine ; Lefebvre, Jean Claude ; Blanchard, Jean Marie ; Singer, Robert H. ; Doglio, Alain. / Terminal minihelix, a novel RNA motif that directs polymerase III transcripts to the cell cytoplasm : Terminal minihelix and RNA export. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 28. pp. 25910-25918.
@article{d21d58841dcc48a1b7682717fe2318e3,
title = "Terminal minihelix, a novel RNA motif that directs polymerase III transcripts to the cell cytoplasm: Terminal minihelix and RNA export",
abstract = "Determining the cis-acting elements controlling nuclear export of RNA is critical, because they specify which RNA will be selected for transport. We have characterized the nuclear export motif of the adenoviral VA1 RNA, a small cytoplasmic RNA transcribed by RNA polymerase III. Using a large panel of VA1 mutants in both transfected COS cells and injected Xenopus oocytes, we showed that the terminal stem of VA1 is necessary and sufficient for its export. Surprisingly, we found that the nucleotide sequence within the terminal stem is not important. Rather, the salient features of this motif are its length and its relative position within the RNA. Such stems thus define a novel and degenerate cytoplasmic localization motif that we termed the minihelix. This motif is found in a variety of polymerase III transcripts, and cross-competition analysis in Xenopus oocytes revealed that export of one such RNA, like hY1 RNA, is specifically competed by VA1 or artificial minihelix. Taken together these results show that the minihelix defines a new cis-acting export element and that this motif could be exported via a novel and specific nuclear export pathway.",
author = "Carole Gwizdek and Edouard Bertrand and Catherine Dargemont and Lefebvre, {Jean Claude} and Blanchard, {Jean Marie} and Singer, {Robert H.} and Alain Doglio",
year = "2001",
month = "7",
day = "13",
doi = "10.1074/jbc.M100493200",
language = "English (US)",
volume = "276",
pages = "25910--25918",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "28",

}

TY - JOUR

T1 - Terminal minihelix, a novel RNA motif that directs polymerase III transcripts to the cell cytoplasm

T2 - Terminal minihelix and RNA export

AU - Gwizdek, Carole

AU - Bertrand, Edouard

AU - Dargemont, Catherine

AU - Lefebvre, Jean Claude

AU - Blanchard, Jean Marie

AU - Singer, Robert H.

AU - Doglio, Alain

PY - 2001/7/13

Y1 - 2001/7/13

N2 - Determining the cis-acting elements controlling nuclear export of RNA is critical, because they specify which RNA will be selected for transport. We have characterized the nuclear export motif of the adenoviral VA1 RNA, a small cytoplasmic RNA transcribed by RNA polymerase III. Using a large panel of VA1 mutants in both transfected COS cells and injected Xenopus oocytes, we showed that the terminal stem of VA1 is necessary and sufficient for its export. Surprisingly, we found that the nucleotide sequence within the terminal stem is not important. Rather, the salient features of this motif are its length and its relative position within the RNA. Such stems thus define a novel and degenerate cytoplasmic localization motif that we termed the minihelix. This motif is found in a variety of polymerase III transcripts, and cross-competition analysis in Xenopus oocytes revealed that export of one such RNA, like hY1 RNA, is specifically competed by VA1 or artificial minihelix. Taken together these results show that the minihelix defines a new cis-acting export element and that this motif could be exported via a novel and specific nuclear export pathway.

AB - Determining the cis-acting elements controlling nuclear export of RNA is critical, because they specify which RNA will be selected for transport. We have characterized the nuclear export motif of the adenoviral VA1 RNA, a small cytoplasmic RNA transcribed by RNA polymerase III. Using a large panel of VA1 mutants in both transfected COS cells and injected Xenopus oocytes, we showed that the terminal stem of VA1 is necessary and sufficient for its export. Surprisingly, we found that the nucleotide sequence within the terminal stem is not important. Rather, the salient features of this motif are its length and its relative position within the RNA. Such stems thus define a novel and degenerate cytoplasmic localization motif that we termed the minihelix. This motif is found in a variety of polymerase III transcripts, and cross-competition analysis in Xenopus oocytes revealed that export of one such RNA, like hY1 RNA, is specifically competed by VA1 or artificial minihelix. Taken together these results show that the minihelix defines a new cis-acting export element and that this motif could be exported via a novel and specific nuclear export pathway.

UR - http://www.scopus.com/inward/record.url?scp=0035854822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035854822&partnerID=8YFLogxK

U2 - 10.1074/jbc.M100493200

DO - 10.1074/jbc.M100493200

M3 - Article

VL - 276

SP - 25910

EP - 25918

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 28

ER -