Tendon-derived stem/progenitor cell aging: Defective self-renewal and altered fate

Zuping Zhou; Takintope Akinbiyi; Lili Xu; Melissa Ramcharan; Daniel J. Leong; Stephen J. Ros; Alexis C. Colvin; Mitchell B. Schaffler; Robert J. Majeska; Evan L. Flatow; Hui B. Sun

doi:10.1111/j.1474-9726.2010.00598.x

Tendon-derived stem/progenitor cell aging: Defective self-renewal and altered fate

Zuping Zhou, Takintope Akinbiyi, Lili Xu, Melissa Ramcharan, Daniel J. Leong, Stephen J. Ros, Alexis C. Colvin, Mitchell B. Schaffler, Robert J. Majeska, Evan L. Flatow, Hui B. Sun

Research output: Contribution to journal › Article › peer-review

154 Scopus citations

Abstract

Aging is a major risk factor for tendon injury and impaired tendon healing, but the basis for these relationships remains poorly understood. Here we show that rat tendon-derived stem/progenitor cells (TSPCs) differ in both self-renewal and differentiation capability with age. The frequency of TSPCs in tendon tissues of aged animals is markedly reduced based on colony formation assays. Proliferation rate is decreased, cell cycle progression is delayed and cell fate patterns are also altered in aged TSPCs. In particular, expression of tendon lineage marker genes is reduced while adipocytic differentiation increased. Cited2, a multi-stimuli responsive transactivator involved in cell growth and senescence, is also downregulated in aged TSPCs while CD44, a matrix assembling and organizing protein implicated in tendon healing, is upregulated, suggesting that these genes participate in the control of TSPC function.

Original language	English (US)
Pages (from-to)	911-915
Number of pages	5
Journal	Aging cell
Volume	9
Issue number	5
DOIs	https://doi.org/10.1111/j.1474-9726.2010.00598.x
State	Published - Oct 2010
Externally published	Yes

Keywords

Aging
CD44
Cited2
Differentiation
Self-renewal
Tendon-derived stem/progenitor cells

ASJC Scopus subject areas

Aging
Cell Biology

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10.1111/j.1474-9726.2010.00598.x

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title = "Tendon-derived stem/progenitor cell aging: Defective self-renewal and altered fate",

abstract = "Aging is a major risk factor for tendon injury and impaired tendon healing, but the basis for these relationships remains poorly understood. Here we show that rat tendon-derived stem/progenitor cells (TSPCs) differ in both self-renewal and differentiation capability with age. The frequency of TSPCs in tendon tissues of aged animals is markedly reduced based on colony formation assays. Proliferation rate is decreased, cell cycle progression is delayed and cell fate patterns are also altered in aged TSPCs. In particular, expression of tendon lineage marker genes is reduced while adipocytic differentiation increased. Cited2, a multi-stimuli responsive transactivator involved in cell growth and senescence, is also downregulated in aged TSPCs while CD44, a matrix assembling and organizing protein implicated in tendon healing, is upregulated, suggesting that these genes participate in the control of TSPC function.",

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author = "Zuping Zhou and Takintope Akinbiyi and Lili Xu and Melissa Ramcharan and Leong, {Daniel J.} and Ros, {Stephen J.} and Colvin, {Alexis C.} and Schaffler, {Mitchell B.} and Majeska, {Robert J.} and Flatow, {Evan L.} and Sun, {Hui B.}",

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AU - Ramcharan, Melissa

AU - Leong, Daniel J.

AU - Ros, Stephen J.

AU - Colvin, Alexis C.

AU - Schaffler, Mitchell B.

AU - Majeska, Robert J.

AU - Flatow, Evan L.

AU - Sun, Hui B.

PY - 2010/10

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AB - Aging is a major risk factor for tendon injury and impaired tendon healing, but the basis for these relationships remains poorly understood. Here we show that rat tendon-derived stem/progenitor cells (TSPCs) differ in both self-renewal and differentiation capability with age. The frequency of TSPCs in tendon tissues of aged animals is markedly reduced based on colony formation assays. Proliferation rate is decreased, cell cycle progression is delayed and cell fate patterns are also altered in aged TSPCs. In particular, expression of tendon lineage marker genes is reduced while adipocytic differentiation increased. Cited2, a multi-stimuli responsive transactivator involved in cell growth and senescence, is also downregulated in aged TSPCs while CD44, a matrix assembling and organizing protein implicated in tendon healing, is upregulated, suggesting that these genes participate in the control of TSPC function.

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Tendon-derived stem/progenitor cell aging: Defective self-renewal and altered fate

Abstract

Keywords

ASJC Scopus subject areas

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