Temporal resolution and sequential expression of muscle-specific genes revealed by in situ hybridization

Jeanne Bentley Lawrence, Krishan Taneja, Robert H. Singer

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The expression of muscle-specific mRNAs was analyzed directly within individual cells by in situ hybridization to chicken skeletal myoblasts undergoing differentiation in vitro. The probes detected mRNAs for sarcomeric myosin heavy chain (MHC) or the skeletal, cardiac, and β isoforms of actin. Precise information as to the expression of these genes in individual cells was obtained and correlated directly with analyses of cell morphology and interactions, cell cycle stage, and immunofluorescence detection of the corresponding proteins. Results demonstrate that mRNAs for the two major muscle-specific proteins, myosin and actin, are not synchronously activated at the time of cell fusion. The mRNA for α-cardiac actin (CAct), known to be the predominant embryonic actin isoform in muscle, is expressed prior to cell fusion and prior to the expression of any isoform of muscle MHC mRNA. MHC mRNA accumulates rapidly immediately after fusion, whereas skeletal actin mRNA is expressed only in larger myofibers. Single cells expressing CAct mRNA have a characteristic short bipolar morphology, are in terminal G1, and do not contain detectable levels of the corresponding protein. In a pattern of expression reciprocal to that of CAct mRNA, β-actin mRNA diminishes to low or undetectable levels in myofibers and in cells of the morphotype which expresses CAct mRNA. Finally, the intracellular distribution of mRNAs for different actin isoforms was compared using nonisotopic detection of isoform-specific oligonucleotide probes. This work illustrates a generally valuable approach to the analysis of cell differentiation and gene expression which directly integrates molecular, morphological, biochemical, and cell cycle information on individual cells.

Original languageEnglish (US)
Pages (from-to)235-246
Number of pages12
JournalDevelopmental Biology
Volume133
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

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In Situ Hybridization
Actins
Muscles
Messenger RNA
Genes
Protein Isoforms
Myosin Heavy Chains
Cell Fusion
Cell Cycle
Skeletal Myoblasts
Gene Expression
Oligonucleotide Probes
Muscle Proteins
Myosins
Cell Communication
Fluorescent Antibody Technique
Cell Differentiation
Chickens
Proteins

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Temporal resolution and sequential expression of muscle-specific genes revealed by in situ hybridization. / Lawrence, Jeanne Bentley; Taneja, Krishan; Singer, Robert H.

In: Developmental Biology, Vol. 133, No. 1, 1989, p. 235-246.

Research output: Contribution to journalArticle

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