Temporal regulation and overlap organization of two Caulobacter flagellar genes

Jeffrey B. Kaplan, Andrew Dingwall, Ruth Bryan, Robert Champer, Lucille Shapiro

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The biogenesis of the bacterial flagellum and chemotaxis apparatus in both Escherichia coli and Caulobacter crescentus requires the ordered expression of over 40 genes whose expression is controlled by a trans-acting regulatory hierarchy. In C. crescentus, additional control mechanisms ensure that the transcription of these genes is initiated at the correct time in the cell cycle. We demonstrate here that two flagellar genes, flaE and fla Y, whose products function in trans to modulate the level of transcription of other flagellar genes, are themselves temporally controlled. DNA sequence analysis of the 3413 base-pairs encompassing the flaE and fla Y coding sequences and the 5′ regulatory region showed that flaE encodes a protein of 16,000 Mr and fla Y a, protein of 17,000 Mr. Evidence that flaE and fla Y are transcribed as a polycistronic message includes (1) the polar effect of Tn5 insertions; (2) deletion analysis showing that the flaE promoter is essential for complementation of both flaE and fla Y alleles; and (3) nuclease S1 assays showing protection of a transcript spanning both genes. The transcript start site in front of flaE was determined and the -10 region conforms to the E. coli sigma 28 promoter consensus sequence. Nuclease S1 analysis also revealed a protected fragment whose size was consistent with a transcript initiating in vivo at a consensus "nif" promoter sequence in front of the fla Y gene. The entire promoter region and an upstream consensus sequence that might be a regulatory element for the fla Y gene lies within the carboxyl-terminal coding sequence of the flaE gene.

Original languageEnglish (US)
Pages (from-to)71-83
Number of pages13
JournalJournal of Molecular Biology
Volume205
Issue number1
DOIs
StatePublished - Jan 5 1989
Externally publishedYes

Fingerprint

Caulobacter
Genes
Caulobacter crescentus
3'-nucleotidase
Consensus Sequence
Escherichia coli
Flagella
Nucleic Acid Regulatory Sequences
Chemotaxis
DNA Sequence Analysis
Genetic Promoter Regions
Base Pairing
Consensus
Cell Cycle
Proteins
Alleles
Gene Expression

ASJC Scopus subject areas

  • Virology

Cite this

Temporal regulation and overlap organization of two Caulobacter flagellar genes. / Kaplan, Jeffrey B.; Dingwall, Andrew; Bryan, Ruth; Champer, Robert; Shapiro, Lucille.

In: Journal of Molecular Biology, Vol. 205, No. 1, 05.01.1989, p. 71-83.

Research output: Contribution to journalArticle

Kaplan, Jeffrey B. ; Dingwall, Andrew ; Bryan, Ruth ; Champer, Robert ; Shapiro, Lucille. / Temporal regulation and overlap organization of two Caulobacter flagellar genes. In: Journal of Molecular Biology. 1989 ; Vol. 205, No. 1. pp. 71-83.
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