Temporal embryonic expression of the sea urchin early H1 gene is controlled by sequences immediately upstream and downstream of the TATA element

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Abstract

The sea urchin early histone H1 gene is expressed only during a very short interval in the life cycle of the organism. In addition to a pool of stored maternal transcripts, the gene is active from the 16-cell stage to the early blastula stage of development, at which time the gene is transcriptionally repressed. We have defined the minimal sequences required for the proper temporal expression of this gene during early embryogenesis. These sequences from -65 to +39 include the TATA element, a conserved sequence CCCACGTACGCAA (part of USE 0) just upstream of the TATA element, an Inr element at the transcription start site, and an internal sequence in the leader region of the H1 transcript. Surprisingly, we could find no role for two highly conserved elements, a GC box (USE I) and an H1-specific element (USE II), that are important in the expression of vertebrate H1 genes. The internal element functions as a positive regulator when three copies are ectopically placed upstream of an early histone H3 TATA box. A temporal regulator is localized within the USE 0 sequence CCCACGTACGCAA, and it apparently plays a role in activation and probably repression of the gene.

Original languageEnglish (US)
Pages (from-to)383-395
Number of pages13
JournalDevelopmental Biology
Volume155
Issue number2
StatePublished - 1993

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Sea Urchins
Genes
Histones
Blastula
TATA Box
Conserved Sequence
Transcription Initiation Site
Life Cycle Stages
Embryonic Development
Vertebrates
Mothers
Gene Expression

ASJC Scopus subject areas

  • Developmental Biology

Cite this

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title = "Temporal embryonic expression of the sea urchin early H1 gene is controlled by sequences immediately upstream and downstream of the TATA element",
abstract = "The sea urchin early histone H1 gene is expressed only during a very short interval in the life cycle of the organism. In addition to a pool of stored maternal transcripts, the gene is active from the 16-cell stage to the early blastula stage of development, at which time the gene is transcriptionally repressed. We have defined the minimal sequences required for the proper temporal expression of this gene during early embryogenesis. These sequences from -65 to +39 include the TATA element, a conserved sequence CCCACGTACGCAA (part of USE 0) just upstream of the TATA element, an Inr element at the transcription start site, and an internal sequence in the leader region of the H1 transcript. Surprisingly, we could find no role for two highly conserved elements, a GC box (USE I) and an H1-specific element (USE II), that are important in the expression of vertebrate H1 genes. The internal element functions as a positive regulator when three copies are ectopically placed upstream of an early histone H3 TATA box. A temporal regulator is localized within the USE 0 sequence CCCACGTACGCAA, and it apparently plays a role in activation and probably repression of the gene.",
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T1 - Temporal embryonic expression of the sea urchin early H1 gene is controlled by sequences immediately upstream and downstream of the TATA element

AU - Fei, Hong

AU - Childs, Geoffrey J.

PY - 1993

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N2 - The sea urchin early histone H1 gene is expressed only during a very short interval in the life cycle of the organism. In addition to a pool of stored maternal transcripts, the gene is active from the 16-cell stage to the early blastula stage of development, at which time the gene is transcriptionally repressed. We have defined the minimal sequences required for the proper temporal expression of this gene during early embryogenesis. These sequences from -65 to +39 include the TATA element, a conserved sequence CCCACGTACGCAA (part of USE 0) just upstream of the TATA element, an Inr element at the transcription start site, and an internal sequence in the leader region of the H1 transcript. Surprisingly, we could find no role for two highly conserved elements, a GC box (USE I) and an H1-specific element (USE II), that are important in the expression of vertebrate H1 genes. The internal element functions as a positive regulator when three copies are ectopically placed upstream of an early histone H3 TATA box. A temporal regulator is localized within the USE 0 sequence CCCACGTACGCAA, and it apparently plays a role in activation and probably repression of the gene.

AB - The sea urchin early histone H1 gene is expressed only during a very short interval in the life cycle of the organism. In addition to a pool of stored maternal transcripts, the gene is active from the 16-cell stage to the early blastula stage of development, at which time the gene is transcriptionally repressed. We have defined the minimal sequences required for the proper temporal expression of this gene during early embryogenesis. These sequences from -65 to +39 include the TATA element, a conserved sequence CCCACGTACGCAA (part of USE 0) just upstream of the TATA element, an Inr element at the transcription start site, and an internal sequence in the leader region of the H1 transcript. Surprisingly, we could find no role for two highly conserved elements, a GC box (USE I) and an H1-specific element (USE II), that are important in the expression of vertebrate H1 genes. The internal element functions as a positive regulator when three copies are ectopically placed upstream of an early histone H3 TATA box. A temporal regulator is localized within the USE 0 sequence CCCACGTACGCAA, and it apparently plays a role in activation and probably repression of the gene.

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