Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development

Philip D. Campbell, Florence L. Marlow

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Homo- and heterodimers of Kif5 proteins form the motor domain of Kinesin-1, a major plus-end directed microtubule motor. Kif5s have been implicated in the intracellular transport of organelles, vesicles, proteins, and RNAs in many cell types. There are three mammalian KIF5s. KIF5A and KIF5C proteins are strictly neural in mouse whereas, KIF5B is ubiquitously expressed. Mouse knockouts indicate crucial roles for KIF5 in development and human mutations in KIF5A lead to the neurodegenerative disease Hereditary Spastic Paraplegia. However, the developmental functions and the extent to which individual kif5 functions overlap have not been elucidated. Zebrafish possess five kif5 genes: kif5Aa, kif5Ab, kif5Ba, kif5Bb, and kif5C. Here we report their tissue specific expression patterns in embryonic and larval stages. Specifically, we find that kif5As are strictly zygotic and exhibit neural-specific expression. In contrast, kif5Bs exhibit strong maternal contribution and are ubiquitously expressed. Lastly, kif5C exhibits weak maternal expression followed by enrichment in neural populations. In addition, kif5s show distinct expression domains in the larval retina.

Original languageEnglish (US)
Pages (from-to)271-279
Number of pages9
JournalGene Expression Patterns
Volume13
Issue number7
DOIs
StatePublished - 2013

Fingerprint

Kinesin
Zebrafish
Gene Expression
Hereditary Spastic Paraplegia
Mothers
Transport Vesicles
Proteins
Hominidae
Human Development
Knockout Mice
Microtubules
Neurodegenerative Diseases
Organelles
Retina
RNA
Mutation
Population
Genes

Keywords

  • Digestive tract
  • Hindbrain
  • Intracellular transport
  • Microtubules
  • Neurons
  • Posterior lateral line ganglia
  • Retina
  • Spinal cord
  • Trigeminal ganglia

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Developmental Biology

Cite this

Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development. / Campbell, Philip D.; Marlow, Florence L.

In: Gene Expression Patterns, Vol. 13, No. 7, 2013, p. 271-279.

Research output: Contribution to journalArticle

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