TY - JOUR
T1 - Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development
AU - Campbell, Philip D.
AU - Marlow, Florence L.
N1 - Funding Information:
We thank Dr. Adrian Santos-Ledo, Dr. Lei Feng, and Xin Li for their valuable comments on the manuscript, Marlow lab members for discussion, and Spartak Kalinin and Clint Depaolo for zebrafish care. Research of the Marlow lab is funded by NIH R01GM1089979 and startup funds to F. Marlow. P. Campbell was supported in part by the Albert Einstein College of Medicine MSTP training grant T32-GM007288.
PY - 2013
Y1 - 2013
N2 - Homo- and heterodimers of Kif5 proteins form the motor domain of Kinesin-1, a major plus-end directed microtubule motor. Kif5s have been implicated in the intracellular transport of organelles, vesicles, proteins, and RNAs in many cell types. There are three mammalian KIF5s. KIF5A and KIF5C proteins are strictly neural in mouse whereas, KIF5B is ubiquitously expressed. Mouse knockouts indicate crucial roles for KIF5 in development and human mutations in KIF5A lead to the neurodegenerative disease Hereditary Spastic Paraplegia. However, the developmental functions and the extent to which individual kif5 functions overlap have not been elucidated. Zebrafish possess five kif5 genes: kif5Aa, kif5Ab, kif5Ba, kif5Bb, and kif5C. Here we report their tissue specific expression patterns in embryonic and larval stages. Specifically, we find that kif5As are strictly zygotic and exhibit neural-specific expression. In contrast, kif5Bs exhibit strong maternal contribution and are ubiquitously expressed. Lastly, kif5C exhibits weak maternal expression followed by enrichment in neural populations. In addition, kif5s show distinct expression domains in the larval retina.
AB - Homo- and heterodimers of Kif5 proteins form the motor domain of Kinesin-1, a major plus-end directed microtubule motor. Kif5s have been implicated in the intracellular transport of organelles, vesicles, proteins, and RNAs in many cell types. There are three mammalian KIF5s. KIF5A and KIF5C proteins are strictly neural in mouse whereas, KIF5B is ubiquitously expressed. Mouse knockouts indicate crucial roles for KIF5 in development and human mutations in KIF5A lead to the neurodegenerative disease Hereditary Spastic Paraplegia. However, the developmental functions and the extent to which individual kif5 functions overlap have not been elucidated. Zebrafish possess five kif5 genes: kif5Aa, kif5Ab, kif5Ba, kif5Bb, and kif5C. Here we report their tissue specific expression patterns in embryonic and larval stages. Specifically, we find that kif5As are strictly zygotic and exhibit neural-specific expression. In contrast, kif5Bs exhibit strong maternal contribution and are ubiquitously expressed. Lastly, kif5C exhibits weak maternal expression followed by enrichment in neural populations. In addition, kif5s show distinct expression domains in the larval retina.
KW - Digestive tract
KW - Hindbrain
KW - Intracellular transport
KW - Microtubules
KW - Neurons
KW - Posterior lateral line ganglia
KW - Retina
KW - Spinal cord
KW - Trigeminal ganglia
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U2 - 10.1016/j.gep.2013.05.002
DO - 10.1016/j.gep.2013.05.002
M3 - Article
C2 - 23684767
AN - SCOPUS:84878884460
SN - 1567-133X
VL - 13
SP - 271
EP - 279
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 7
ER -