Temperature-sensitive mutants of reovirus type 3

Defects in viral maturation as studied by immunofluorescence and electron microscopy

Bernard N. Fields, Cedric S. Raine, Stephen G. Baum

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Studies using electron microscopy and immunofluorescence have been performed on cells infected with five different groups of temperature-sensitive mutants of reovirus type 3. Mutants of groups A and E induce normal appearing viral factories. Mutants of group B induce factories which accumulate viral cores, while factories of groups C and D accumulate empty viral capsids. It is suggested that the latter three genetic groups consist of mutants with defects in virion assembly.

Original languageEnglish (US)
Pages (from-to)569-578
Number of pages10
JournalVirology
Volume43
Issue number3
DOIs
StatePublished - 1971

Fingerprint

Mammalian orthoreovirus 3
Capsid
Fluorescence Microscopy
Virion
Fluorescent Antibody Technique
Electron Microscopy
Temperature

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Temperature-sensitive mutants of reovirus type 3 : Defects in viral maturation as studied by immunofluorescence and electron microscopy. / Fields, Bernard N.; Raine, Cedric S.; Baum, Stephen G.

In: Virology, Vol. 43, No. 3, 1971, p. 569-578.

Research output: Contribution to journalArticle

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