Temperature-induced conformational changes in prosomatostatin-II: Implications for processing

Joydeep Mitra, Xue Jun Tang, Steven C. Almo, Dennis Shields

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Somatostatin (SRIF) is a 14-residue peptide hormone synthesized in the hypothalamus and pancreatic islets. SRIF-14 and an N-terminally extended form, SRIF-28, are generated by the proteolytic processing of an approx. 102-residue precursor prosomatostatin (proSRIF) at a single set of paired basic residues (Arg-Lys) and at a monobasic (Arg) site respectively. Previous work in our laboratory demonstrated that the propeptide of SRIF mediates intracellular sorting; we suggested that this information resides in the prohormone structure. To identify putative sorting domains we have investigated structural features of recombinant anglerfish proSRIF-II purified from Escherichia coli. Two species of proSRIF-II were obtained: a monomeric form and a disulphide-linked dimer. CD analyses revealed that monomeric proSRIF-II lacks appreciable periodic secondary structure; however, on slow heating (2°C/min) and cooling, it assumed a predominantly α-helical conformation. When subjected to a second heating-and-cooling cycle, the α-helical conformation was maintained. In contrast, the dimeric form of proSRIF-II was predominantly α-helical and its helicity did not increase in response to heating and recooling. Our results suggest that proSRIF-II might exist in several different folding intermediate states.

Original languageEnglish (US)
Pages (from-to)275-282
Number of pages8
JournalBiochemical Journal
Volume334
Issue number1
StatePublished - Aug 15 1998

Fingerprint

Heating
Sorting
Temperature
arginyllysine
Conformations
Processing
Cooling
Peptide Hormones
Somatostatin
Islets of Langerhans
Disulfides
Dimers
Escherichia coli
Hypothalamus
prosomatostatin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Temperature-induced conformational changes in prosomatostatin-II : Implications for processing. / Mitra, Joydeep; Tang, Xue Jun; Almo, Steven C.; Shields, Dennis.

In: Biochemical Journal, Vol. 334, No. 1, 15.08.1998, p. 275-282.

Research output: Contribution to journalArticle

Mitra, Joydeep ; Tang, Xue Jun ; Almo, Steven C. ; Shields, Dennis. / Temperature-induced conformational changes in prosomatostatin-II : Implications for processing. In: Biochemical Journal. 1998 ; Vol. 334, No. 1. pp. 275-282.
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