Temperature dependence of the toxic effects of phenytoin on peripheral neuromuscular function of the rat tail

Joaquín Romá, Ana Maria Cuervo, Fernando Macian-Juan, Angel Raya, Juan Gallego, J. Emilio Llopis, Francisco J. Romero

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We studied the acute effects of a single dose of phenytoin (250 mg/kg) on peripheral neuromuscular function. The evoked muscle action potentials of the dorsal segmental muscles in the rat tail, and the conduction velocity of the dorsal nerve trunk which innervates them, were measured before and after the intraperitoneal injection of phenytoin. The experiments were performed at different temperatures, 27 (physiological tail temperature), 36 and 37°C (physiological central temperature) in different groups of animals. The amplitudes of the evoked muscle action potentials in the treated groups showed no significant modifications at 27°C, at 36°C a small nonsignificant decrease could be observed, and a complete block occurred at 37°C. The mean blocking time was approximately one hour. No significant variations of conduction velocity were observed at 27 and 36°C, whereas it decreased significantly after 30 minutes at 37°C. The results presented confirm phenytoin toxicity. How far these results, especially the decrease of nerve conduction velocity observed at 37°C, confirm a previous hypothesis which supported that peripheral and central nervous system are affected by phenytoin by similar mechanisms, is discussed.

Original languageEnglish (US)
Pages (from-to)627-631
Number of pages5
JournalNeurotoxicology and Teratology
Volume12
Issue number6
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Poisons
Phenytoin
Tail
Rats
Muscle
Temperature
Muscles
Action Potentials
Neural Conduction
Peripheral Nervous System
Neurology
Intraperitoneal Injections
Toxicity
Animals
Central Nervous System
Experiments

Keywords

  • Motor nerve conduction velocity
  • Muscle action potential
  • Peripheral neuromuscular function
  • Phenytoin
  • Rat tail
  • Temperature dependence
  • Toxicity

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience
  • Toxicology

Cite this

Temperature dependence of the toxic effects of phenytoin on peripheral neuromuscular function of the rat tail. / Romá, Joaquín; Cuervo, Ana Maria; Macian-Juan, Fernando; Raya, Angel; Gallego, Juan; Llopis, J. Emilio; Romero, Francisco J.

In: Neurotoxicology and Teratology, Vol. 12, No. 6, 1990, p. 627-631.

Research output: Contribution to journalArticle

Romá, Joaquín ; Cuervo, Ana Maria ; Macian-Juan, Fernando ; Raya, Angel ; Gallego, Juan ; Llopis, J. Emilio ; Romero, Francisco J. / Temperature dependence of the toxic effects of phenytoin on peripheral neuromuscular function of the rat tail. In: Neurotoxicology and Teratology. 1990 ; Vol. 12, No. 6. pp. 627-631.
@article{f0af86e88d43401db570f2b1c46f2c42,
title = "Temperature dependence of the toxic effects of phenytoin on peripheral neuromuscular function of the rat tail",
abstract = "We studied the acute effects of a single dose of phenytoin (250 mg/kg) on peripheral neuromuscular function. The evoked muscle action potentials of the dorsal segmental muscles in the rat tail, and the conduction velocity of the dorsal nerve trunk which innervates them, were measured before and after the intraperitoneal injection of phenytoin. The experiments were performed at different temperatures, 27 (physiological tail temperature), 36 and 37°C (physiological central temperature) in different groups of animals. The amplitudes of the evoked muscle action potentials in the treated groups showed no significant modifications at 27°C, at 36°C a small nonsignificant decrease could be observed, and a complete block occurred at 37°C. The mean blocking time was approximately one hour. No significant variations of conduction velocity were observed at 27 and 36°C, whereas it decreased significantly after 30 minutes at 37°C. The results presented confirm phenytoin toxicity. How far these results, especially the decrease of nerve conduction velocity observed at 37°C, confirm a previous hypothesis which supported that peripheral and central nervous system are affected by phenytoin by similar mechanisms, is discussed.",
keywords = "Motor nerve conduction velocity, Muscle action potential, Peripheral neuromuscular function, Phenytoin, Rat tail, Temperature dependence, Toxicity",
author = "Joaqu{\'i}n Rom{\'a} and Cuervo, {Ana Maria} and Fernando Macian-Juan and Angel Raya and Juan Gallego and Llopis, {J. Emilio} and Romero, {Francisco J.}",
year = "1990",
doi = "10.1016/0892-0362(90)90075-N",
language = "English (US)",
volume = "12",
pages = "627--631",
journal = "Neurotoxicology and Teratology",
issn = "0892-0362",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Temperature dependence of the toxic effects of phenytoin on peripheral neuromuscular function of the rat tail

AU - Romá, Joaquín

AU - Cuervo, Ana Maria

AU - Macian-Juan, Fernando

AU - Raya, Angel

AU - Gallego, Juan

AU - Llopis, J. Emilio

AU - Romero, Francisco J.

PY - 1990

Y1 - 1990

N2 - We studied the acute effects of a single dose of phenytoin (250 mg/kg) on peripheral neuromuscular function. The evoked muscle action potentials of the dorsal segmental muscles in the rat tail, and the conduction velocity of the dorsal nerve trunk which innervates them, were measured before and after the intraperitoneal injection of phenytoin. The experiments were performed at different temperatures, 27 (physiological tail temperature), 36 and 37°C (physiological central temperature) in different groups of animals. The amplitudes of the evoked muscle action potentials in the treated groups showed no significant modifications at 27°C, at 36°C a small nonsignificant decrease could be observed, and a complete block occurred at 37°C. The mean blocking time was approximately one hour. No significant variations of conduction velocity were observed at 27 and 36°C, whereas it decreased significantly after 30 minutes at 37°C. The results presented confirm phenytoin toxicity. How far these results, especially the decrease of nerve conduction velocity observed at 37°C, confirm a previous hypothesis which supported that peripheral and central nervous system are affected by phenytoin by similar mechanisms, is discussed.

AB - We studied the acute effects of a single dose of phenytoin (250 mg/kg) on peripheral neuromuscular function. The evoked muscle action potentials of the dorsal segmental muscles in the rat tail, and the conduction velocity of the dorsal nerve trunk which innervates them, were measured before and after the intraperitoneal injection of phenytoin. The experiments were performed at different temperatures, 27 (physiological tail temperature), 36 and 37°C (physiological central temperature) in different groups of animals. The amplitudes of the evoked muscle action potentials in the treated groups showed no significant modifications at 27°C, at 36°C a small nonsignificant decrease could be observed, and a complete block occurred at 37°C. The mean blocking time was approximately one hour. No significant variations of conduction velocity were observed at 27 and 36°C, whereas it decreased significantly after 30 minutes at 37°C. The results presented confirm phenytoin toxicity. How far these results, especially the decrease of nerve conduction velocity observed at 37°C, confirm a previous hypothesis which supported that peripheral and central nervous system are affected by phenytoin by similar mechanisms, is discussed.

KW - Motor nerve conduction velocity

KW - Muscle action potential

KW - Peripheral neuromuscular function

KW - Phenytoin

KW - Rat tail

KW - Temperature dependence

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=0025165650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025165650&partnerID=8YFLogxK

U2 - 10.1016/0892-0362(90)90075-N

DO - 10.1016/0892-0362(90)90075-N

M3 - Article

VL - 12

SP - 627

EP - 631

JO - Neurotoxicology and Teratology

JF - Neurotoxicology and Teratology

SN - 0892-0362

IS - 6

ER -