Telomerase

structure and properties of the enzyme, characteristics of the yeast telomerase

Daria Shcherbakova, M. E. Zvereva, O. V. Shpanchenko, O. A. Dontsova

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Telomerase is a ribonucleoprotein that extends the telomeric ends of the chromosomes to counterbalance the natural shortening due to incomplete DNA replication in eukaryotic cells. The core enzyme consists of catalytic reverse transcriptase subunit TERT (Telomerase Reverse Transcriptase) and RNA subunit TER (Telomerase RNA), a short specific region of which serves as a template for synthesis of the telomeric repeats. In this review we focus on the telomerase from yeast Saccharomyces cerevisiae. Despite the intensive research of telomerase in different organisms, the enzyme mechanism remains unclear. The observed peculiarities of the yeast telomerase is of great interest too. Unlike ciliate and human telomerases, yeast enzyme can add only one telomeric repeat to a DNA oligonucleotide (primer) imitating the single-stranded telomeric end of the chromosome and remains stably bound to it after elongation. This review is an attempt to summarise results of numerous studies of the structure and functions of the core enzyme components, their interactions between each other and with a primer, telomerase activity on different substrates in vitro. Also the peculiarities of the telomerase functioning in a cell and accessory proteins of the telomerase complex are discussed.

Original languageEnglish (US)
Pages (from-to)580-594
Number of pages15
JournalMolekulyarnaya Biologiya
Volume40
Issue number4
StatePublished - Jul 2006
Externally publishedYes

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Telomerase
Yeasts
Enzymes
DNA Primers
Chromosomes
Ribonucleoproteins
RNA-Directed DNA Polymerase
Eukaryotic Cells
DNA Replication
Saccharomyces cerevisiae
RNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Telomerase : structure and properties of the enzyme, characteristics of the yeast telomerase. / Shcherbakova, Daria; Zvereva, M. E.; Shpanchenko, O. V.; Dontsova, O. A.

In: Molekulyarnaya Biologiya, Vol. 40, No. 4, 07.2006, p. 580-594.

Research output: Contribution to journalArticle

Shcherbakova, Daria ; Zvereva, M. E. ; Shpanchenko, O. V. ; Dontsova, O. A. / Telomerase : structure and properties of the enzyme, characteristics of the yeast telomerase. In: Molekulyarnaya Biologiya. 2006 ; Vol. 40, No. 4. pp. 580-594.
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