Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer

D. M. Shcherbakova; K. A. Sokolov; M. I. Zvereva; O. A. Dontsova

doi:10.1134/S0006297909070074

Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer

D. M. Shcherbakova, K. A. Sokolov, M. I. Zvereva, O. A. Dontsova

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. Our results indicate that yeast telomerase is active in vitro as a monomer.

Original language	English (US)
Pages (from-to)	749-755
Number of pages	7
Journal	Biochemistry (Moscow)
Volume	74
Issue number	7
DOIs	https://doi.org/10.1134/S0006297909070074
State	Published - Jul 2009
Externally published	Yes

Keywords

RNA aptamer
Telomerase dimerization
Yeast telomerase

ASJC Scopus subject areas

Biochemistry

Access to Document

10.1134/S0006297909070074

Cite this

@article{61855095818147238a04292906364575,

title = "Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer",

abstract = "A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. Our results indicate that yeast telomerase is active in vitro as a monomer.",

keywords = "RNA aptamer, Telomerase dimerization, Yeast telomerase",

author = "Shcherbakova, {D. M.} and Sokolov, {K. A.} and Zvereva, {M. I.} and Dontsova, {O. A.}",

note = "Funding Information: This work was supported by the Human Frontier Science Program Organization (RGP 0032/2005), Howard Hughes Medical Institute (55005605), and the Russian Foundation for Basic Research (grants 08 04 01220 a and 07 04 92119 a).",

year = "2009",

month = jul,

doi = "10.1134/S0006297909070074",

language = "English (US)",

volume = "74",

pages = "749--755",

journal = "Biochemistry (Moscow)",

issn = "0006-2979",

publisher = "Maik Nauka-Interperiodica Publishing",

number = "7",

}

TY - JOUR

T1 - Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer

AU - Shcherbakova, D. M.

AU - Sokolov, K. A.

AU - Zvereva, M. I.

AU - Dontsova, O. A.

N1 - Funding Information: This work was supported by the Human Frontier Science Program Organization (RGP 0032/2005), Howard Hughes Medical Institute (55005605), and the Russian Foundation for Basic Research (grants 08 04 01220 a and 07 04 92119 a).

PY - 2009/7

Y1 - 2009/7

N2 - A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. Our results indicate that yeast telomerase is active in vitro as a monomer.

AB - A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. Our results indicate that yeast telomerase is active in vitro as a monomer.

KW - RNA aptamer

KW - Telomerase dimerization

KW - Yeast telomerase

UR - http://www.scopus.com/inward/record.url?scp=68349106667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68349106667&partnerID=8YFLogxK

U2 - 10.1134/S0006297909070074

DO - 10.1134/S0006297909070074

M3 - Article

C2 - 19747095

AN - SCOPUS:68349106667

SN - 0006-2979

VL - 74

SP - 749

EP - 755

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 7

ER -

Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this