Telomerase from yeast Saccharomyces cerevisiae is active in vitro as a monomer

D. M. Shcherbakova, K. A. Sokolov, M. I. Zvereva, O. A. Dontsova

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A system for isolation of yeast telomerase via RNA affinity tag in TLC1 RNA was developed. Streptavidin aptamer was inserted at two different positions in TLC1 RNA. Telomerase with TLC1 RNA with one of these inserts is functional in vivo and can be isolated by affinity chromatography in vitro. A telomerase preparation isolated using this technique from a strain producing two distinguishable TLC1 RNA molecules (with and without aptameric insertion) resulted in isolation of active telomerase containing only TLC1 RNA with the aptamer. Our results indicate that yeast telomerase is active in vitro as a monomer.

Original languageEnglish (US)
Pages (from-to)749-755
Number of pages7
JournalBiochemistry (Moscow)
Volume74
Issue number7
DOIs
Publication statusPublished - Jul 1 2009
Externally publishedYes

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Keywords

  • RNA aptamer
  • Telomerase dimerization
  • Yeast telomerase

ASJC Scopus subject areas

  • Biochemistry

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