Techniques to study epigenetic control and the epigenome in parasites

Sheila C. Nardelli, Li Min Ting, Kami Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Epigenetics is the study of heritable changes in gene expression that occur independent of the DNA sequence. Due to their intimacy with DNA, histones have a central role in chromatin structure and epigenetic regulation. Their tails are subject to posttranslational modifications (PTMs) that together with chromatin- remodeling proteins control the access of different proteins to DNA and allow a precise response to different environmental conditions. The first part of this chapter is dedicated to histone enrichment methods that allow the study of histones using techniques such as immunoblot or mass spectrometry for the mapping of the histone PTM network. Next we describe chromatin immunoprecipitation-based techniques (ChIP) for study of the epigenome. ChIP followed by microarray or next-generation sequencing enables the precise genomic localization of protein-DNA interactions. These techniques for genomewide profiling of chromatin provide powerful and efficient tools to study the epigenome.

Original languageEnglish (US)
Title of host publicationParasite Genomics Protocols: Second Edition
PublisherSpringer Fachmedien
Pages177-191
Number of pages15
ISBN (Print)9781493914388, 9781493914371
DOIs
Publication statusPublished - Nov 11 2014

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Keywords

  • Chromatin immunoprecipitation
  • Histone
  • Microarray
  • Sequencing

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Nardelli, S. C., Ting, L. M., & Kim, K. (2014). Techniques to study epigenetic control and the epigenome in parasites. In Parasite Genomics Protocols: Second Edition (pp. 177-191). Springer Fachmedien. https://doi.org/10.1007/978-1-4939-1438-8_10