Techniques to study epigenetic control and the epigenome in parasites

Sheila C. Nardelli, Li Min Ting, Kami Kim

Research output: Contribution to journalArticle

Abstract

Epigenetics is the study of heritable changes in gene expression that occur independent of the DNA sequence. Due to their intimacy with DNA, histones have a central role in chromatin structure and epigenetic regulation. Their tails are subject to posttranslational modifications (PTMs) that together with chromatin- remodeling proteins control the access of different proteins to DNA and allow a precise response to different environmental conditions. The first part of this chapter is dedicated to histone enrichment methods that allow the study of histones using techniques such as immunoblot or mass spectrometry for the mapping of the histone PTM network. Next we describe chromatin immunoprecipitation-based techniques (ChIP) for study of the epigenome. ChIP followed by microarray or next-generation sequencing enables the precise genomic localization of protein-DNA interactions. These techniques for genomewide profiling of chromatin provide powerful and efficient tools to study the epigenome.

Original languageEnglish (US)
Pages (from-to)177-191
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume1201
DOIs
Publication statusPublished - 2015
Externally publishedYes

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Keywords

  • Chromatin immunoprecipitation
  • Histone
  • Microarray
  • Sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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