Taxol stabilizes microtubules in mouse fibroblast cells

P. B. Schiff, Susan Band Horwitz

Research output: Contribution to journalArticle

1616 Citations (Scopus)

Abstract

Taxol, a potent inhibitor of human HeLa and mouse fibroblast cell replication, blocked cells in the G2 and M phase of the cell cycle and stabilized cytoplasmic microtubules. The cytoplasmic microtubules of taxol-treated cells were visualized by transmission electron microscopy and indirect immunofluorescence microscopy. More than 90% of the cells treated with 10 μM taxol for 22 hr at 37°C displayed bundles of microtubules that appeared to radiate from a common site (or sites), in addition to their cytoplasmic microtubules. Untreated cells that were kept in the cold (4°C) for 16 hr lost their microtubules, whereas cells that were pretreated with taxol for 22 hr at 37°C continued to display their microtubules and bundles of microtubules in the cold. Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.

Original languageEnglish (US)
Pages (from-to)1561-1565
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number3 I
StatePublished - 1980

Fingerprint

Paclitaxel
Microtubules
Fibroblasts
Pseudopodia
Aptitude
G2 Phase
Indirect Fluorescent Antibody Technique
Transmission Electron Microscopy
Fluorescence Microscopy
Cell Division
Cell Cycle

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Taxol stabilizes microtubules in mouse fibroblast cells. / Schiff, P. B.; Band Horwitz, Susan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 3 I, 1980, p. 1561-1565.

Research output: Contribution to journalArticle

@article{3c39cb3b1c8845bfb16af09a6ac275dd,
title = "Taxol stabilizes microtubules in mouse fibroblast cells",
abstract = "Taxol, a potent inhibitor of human HeLa and mouse fibroblast cell replication, blocked cells in the G2 and M phase of the cell cycle and stabilized cytoplasmic microtubules. The cytoplasmic microtubules of taxol-treated cells were visualized by transmission electron microscopy and indirect immunofluorescence microscopy. More than 90{\%} of the cells treated with 10 μM taxol for 22 hr at 37°C displayed bundles of microtubules that appeared to radiate from a common site (or sites), in addition to their cytoplasmic microtubules. Untreated cells that were kept in the cold (4°C) for 16 hr lost their microtubules, whereas cells that were pretreated with taxol for 22 hr at 37°C continued to display their microtubules and bundles of microtubules in the cold. Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.",
author = "Schiff, {P. B.} and {Band Horwitz}, Susan",
year = "1980",
language = "English (US)",
volume = "77",
pages = "1561--1565",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "3 I",

}

TY - JOUR

T1 - Taxol stabilizes microtubules in mouse fibroblast cells

AU - Schiff, P. B.

AU - Band Horwitz, Susan

PY - 1980

Y1 - 1980

N2 - Taxol, a potent inhibitor of human HeLa and mouse fibroblast cell replication, blocked cells in the G2 and M phase of the cell cycle and stabilized cytoplasmic microtubules. The cytoplasmic microtubules of taxol-treated cells were visualized by transmission electron microscopy and indirect immunofluorescence microscopy. More than 90% of the cells treated with 10 μM taxol for 22 hr at 37°C displayed bundles of microtubules that appeared to radiate from a common site (or sites), in addition to their cytoplasmic microtubules. Untreated cells that were kept in the cold (4°C) for 16 hr lost their microtubules, whereas cells that were pretreated with taxol for 22 hr at 37°C continued to display their microtubules and bundles of microtubules in the cold. Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.

AB - Taxol, a potent inhibitor of human HeLa and mouse fibroblast cell replication, blocked cells in the G2 and M phase of the cell cycle and stabilized cytoplasmic microtubules. The cytoplasmic microtubules of taxol-treated cells were visualized by transmission electron microscopy and indirect immunofluorescence microscopy. More than 90% of the cells treated with 10 μM taxol for 22 hr at 37°C displayed bundles of microtubules that appeared to radiate from a common site (or sites), in addition to their cytoplasmic microtubules. Untreated cells that were kept in the cold (4°C) for 16 hr lost their microtubules, whereas cells that were pretreated with taxol for 22 hr at 37°C continued to display their microtubules and bundles of microtubules in the cold. Taxol inhibited the migration behavior of fibroblast cells, but these cells did not lose their ability to produce mobile surface projections such as lamellipodia and filopodia.

UR - http://www.scopus.com/inward/record.url?scp=0000297058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000297058&partnerID=8YFLogxK

M3 - Article

C2 - 6103535

AN - SCOPUS:0000297058

VL - 77

SP - 1561

EP - 1565

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 3 I

ER -