Targeted RNA editing: novel tools to study post-transcriptional regulation

Weijin Xu, Jeetayu Biswas, Robert H. Singer, Michael Rosbash

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations

Abstract

RNA binding proteins (RBPs) regulate nearly all post-transcriptional processes within cells. To fully understand RBP function, it is essential to identify their in vivo targets. Standard techniques for profiling RBP targets, such as crosslinking immunoprecipitation (CLIP) and its variants, are limited or suboptimal in some situations, e.g. when compatible antibodies are not available and when dealing with small cell populations such as neuronal subtypes and primary stem cells. This review summarizes and compares several genetic approaches recently designed to identify RBP targets in such circumstances. TRIBE (targets of RNA binding proteins identified by editing), RNA tagging, and STAMP (surveying targets by APOBEC-mediated profiling) are new genetic tools useful for the study of post-transcriptional regulation and RBP identification. We describe the underlying RNA base editing technology, recent applications, and therapeutic implications.

Original languageEnglish (US)
Pages (from-to)389-403
Number of pages15
JournalMolecular Cell
Volume82
Issue number2
DOIs
StatePublished - Jan 20 2022

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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