TY - JOUR
T1 - Targeted RNA editing
T2 - novel tools to study post-transcriptional regulation
AU - Xu, Weijin
AU - Biswas, Jeetayu
AU - Singer, Robert H.
AU - Rosbash, Michael
N1 - Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2022/1/20
Y1 - 2022/1/20
N2 - RNA binding proteins (RBPs) regulate nearly all post-transcriptional processes within cells. To fully understand RBP function, it is essential to identify their in vivo targets. Standard techniques for profiling RBP targets, such as crosslinking immunoprecipitation (CLIP) and its variants, are limited or suboptimal in some situations, e.g. when compatible antibodies are not available and when dealing with small cell populations such as neuronal subtypes and primary stem cells. This review summarizes and compares several genetic approaches recently designed to identify RBP targets in such circumstances. TRIBE (targets of RNA binding proteins identified by editing), RNA tagging, and STAMP (surveying targets by APOBEC-mediated profiling) are new genetic tools useful for the study of post-transcriptional regulation and RBP identification. We describe the underlying RNA base editing technology, recent applications, and therapeutic implications.
AB - RNA binding proteins (RBPs) regulate nearly all post-transcriptional processes within cells. To fully understand RBP function, it is essential to identify their in vivo targets. Standard techniques for profiling RBP targets, such as crosslinking immunoprecipitation (CLIP) and its variants, are limited or suboptimal in some situations, e.g. when compatible antibodies are not available and when dealing with small cell populations such as neuronal subtypes and primary stem cells. This review summarizes and compares several genetic approaches recently designed to identify RBP targets in such circumstances. TRIBE (targets of RNA binding proteins identified by editing), RNA tagging, and STAMP (surveying targets by APOBEC-mediated profiling) are new genetic tools useful for the study of post-transcriptional regulation and RBP identification. We describe the underlying RNA base editing technology, recent applications, and therapeutic implications.
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U2 - 10.1016/j.molcel.2021.10.010
DO - 10.1016/j.molcel.2021.10.010
M3 - Review article
C2 - 34739873
AN - SCOPUS:85122991202
SN - 1097-2765
VL - 82
SP - 389
EP - 403
JO - Molecular Cell
JF - Molecular Cell
IS - 2
ER -