Target cell susceptibility to lysis by human natural killer cells is augmented by α(1,3)-galactosyltransferase and reduced by α(1,2)- fucosyltransferase

John H. Artrip, Pawel Kwiatkowski, Robert E. Michler, Shu Feng Wang, Sorina Tugulea, Jan Ankersmit, Larisa Chisholm, Ian F C McKenzie, Mauro S. Sandrin, Silviu Itescu

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Susceptibility of porcine endothelial cells to human natural killer (NK) cell lysis was found to reflect surface expression of ligands containing Gal α(1,3)GlcNAc, the principal antigen on porcine endothelium recognized by xenoreactive human antibodies. Genetically modifying expression of this epitope on porcine endothelium by transfection with the α(1,2)- fucosyltransferase gene reduced susceptibility to human NK lysis. These results indicate that surface carbohydrate remodeling profoundly affects target cell susceptibility to NK lysis, and suggest that successful transgenic strategies to limit xenograft rejection by NK cells and xenoreactive antibodies will need to incorporate carbohydrate remodeling.

Original languageEnglish (US)
Pages (from-to)10717-10722
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number16
DOIs
StatePublished - Apr 16 1999
Externally publishedYes

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Galactosyltransferases
Natural Killer Cells
Swine
Carbohydrates
Endothelium
Antibodies
Endothelial cells
Heterografts
Epitopes
Genes
Ligands
Antigens
Transfection
Endothelial Cells
galactoside 2-alpha-L-fucosyltransferase
galactosyl-1,3-N-acetylglucosamine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Target cell susceptibility to lysis by human natural killer cells is augmented by α(1,3)-galactosyltransferase and reduced by α(1,2)- fucosyltransferase. / Artrip, John H.; Kwiatkowski, Pawel; Michler, Robert E.; Wang, Shu Feng; Tugulea, Sorina; Ankersmit, Jan; Chisholm, Larisa; McKenzie, Ian F C; Sandrin, Mauro S.; Itescu, Silviu.

In: Journal of Biological Chemistry, Vol. 274, No. 16, 16.04.1999, p. 10717-10722.

Research output: Contribution to journalArticle

Artrip, JH, Kwiatkowski, P, Michler, RE, Wang, SF, Tugulea, S, Ankersmit, J, Chisholm, L, McKenzie, IFC, Sandrin, MS & Itescu, S 1999, 'Target cell susceptibility to lysis by human natural killer cells is augmented by α(1,3)-galactosyltransferase and reduced by α(1,2)- fucosyltransferase', Journal of Biological Chemistry, vol. 274, no. 16, pp. 10717-10722. https://doi.org/10.1074/jbc.274.16.10717
Artrip, John H. ; Kwiatkowski, Pawel ; Michler, Robert E. ; Wang, Shu Feng ; Tugulea, Sorina ; Ankersmit, Jan ; Chisholm, Larisa ; McKenzie, Ian F C ; Sandrin, Mauro S. ; Itescu, Silviu. / Target cell susceptibility to lysis by human natural killer cells is augmented by α(1,3)-galactosyltransferase and reduced by α(1,2)- fucosyltransferase. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 16. pp. 10717-10722.
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abstract = "Susceptibility of porcine endothelial cells to human natural killer (NK) cell lysis was found to reflect surface expression of ligands containing Gal α(1,3)GlcNAc, the principal antigen on porcine endothelium recognized by xenoreactive human antibodies. Genetically modifying expression of this epitope on porcine endothelium by transfection with the α(1,2)- fucosyltransferase gene reduced susceptibility to human NK lysis. These results indicate that surface carbohydrate remodeling profoundly affects target cell susceptibility to NK lysis, and suggest that successful transgenic strategies to limit xenograft rejection by NK cells and xenoreactive antibodies will need to incorporate carbohydrate remodeling.",
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AU - Wang, Shu Feng

AU - Tugulea, Sorina

AU - Ankersmit, Jan

AU - Chisholm, Larisa

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AU - Sandrin, Mauro S.

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