Tapasin enhances assembly of transporters associated with antigen processing-dependent and -independent peptides with HLA-A2 and HLA-B27 expressed in insect cells

Gregoire Lauvau, Brigitte Gubler, Hélène Cohen, Soizic Daniel, Sophie Caillat-Zucman, Peter M. Van Endert

Research output: Contribution to journalArticle

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Abstract

Assembly of HLA class I-peptide complexes is assisted by multiple proteins that associate with HLa molecules in loading complexes. These include the housekeeping chaperones calnexin and calreticulin and two essential proteins, the transporters associated with antigen processing (TAP) for peptide supply, and the protein tapasin which is thought to act as a specialized chaperone. We dissected functional effects of processing cofactors by co-expressing in insect cells various combinations of the human proteins HLA-A2, HLA-B27, β2-microglobulin, TAP, calnexin, calreticulin, and tapasin. Stability at 37 °C and surface expression of class I dimers correlated closely in baculovirus-infected Sf9 cells, suggesting that these cells retain empty dimers in the endoplasmic reticulum. Both HLA molecules form substantial quantities of stable complexes with insect cell-produced peptide pools. These pools are TAP-selected cytosolic peptides for HLA-B27 but endoplasmic reticulum-derived, i.e. TAP-independent peptides for HLA-A2. This discrepancy may be due to peptide selection by human TAP which is much better adapted to the HLA-B27 than to the HLA-A2 ligand preferences. HLA class I assembly with peptides from TAP-dependent and -independent pools was enhanced strongly by tapasin. Thus, tapasin acts as a chaperone and/or peptide editor that facilitates assembly of peptides with HLA class I molecules independently of mediating their interaction with TAP and/or retention in the endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)31349-31358
Number of pages10
JournalJournal of Biological Chemistry
Volume274
Issue number44
DOIs
StatePublished - Oct 29 1999
Externally publishedYes

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HLA-A2 Antigen
HLA-B27 Antigen
Antigen Presentation
trypsinogen activation peptide
Insects
Antigens
Peptides
Processing
Calnexin
Endoplasmic Reticulum
Calreticulin
Dimers
Proteins
Molecules
Sf9 Cells
Housekeeping
Baculoviridae
tapasin
Ligands

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tapasin enhances assembly of transporters associated with antigen processing-dependent and -independent peptides with HLA-A2 and HLA-B27 expressed in insect cells. / Lauvau, Gregoire; Gubler, Brigitte; Cohen, Hélène; Daniel, Soizic; Caillat-Zucman, Sophie; Van Endert, Peter M.

In: Journal of Biological Chemistry, Vol. 274, No. 44, 29.10.1999, p. 31349-31358.

Research output: Contribution to journalArticle

Lauvau, Gregoire ; Gubler, Brigitte ; Cohen, Hélène ; Daniel, Soizic ; Caillat-Zucman, Sophie ; Van Endert, Peter M. / Tapasin enhances assembly of transporters associated with antigen processing-dependent and -independent peptides with HLA-A2 and HLA-B27 expressed in insect cells. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 44. pp. 31349-31358.
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