Abstract
Objective: To assess the detection and quantitation of HIV-1 from tampon eluents in comparison with cervicovaginal lavage (CVL) and plasma specimens from the same women. Methods: Ninety-seven tampon, 105 CVL, and 104 plasma specimens from 105 HIV-1 seropositive women were analyzed using Version 3 of the Chiron bDNA assay, with sensitivity of 50 HIV-1 RNA copies/ml. Data analyses used McNemar's test, Wilcoxon signed rank test, and Mantel-Haenszel chi-squared and odds ratios with 95% confidence intervals to assess differences in proportions. Results: In women for whom both plasma and genital specimens were available, HIV-1 was detected less frequently in genital specimens: [tampons (33/97, 34%) and CVL (48/104, 46%)] than plasma specimens (86/104, 83%) (P< 0.001 for both plasma versus tampon and for plasma versus CVL). However, the proportion of genital specimens with detectable virus did not differ significantly by collection method (P = 0.14). Among women with detectable virus using both collection methods (n = 23), viral load was similar for tampon eluents (median, 355 copies/ml; range, 52-120898) and CVL specimens (median, 265 copies/ml; range, 61-35637; P = 0.88). Conclusion: Tampon eluent specimens are slightly less sensitive than CVL specimens in the detection of genital HIV-1, although quantification of viral load, when detectable by both methods, was similar.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1417-1420 |
| Number of pages | 4 |
| Journal | AIDS |
| Volume | 15 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jul 27 2001 |
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Keywords
- Cervicovaginal lavage
- Genital HIV-1
- Tampon
- Viral load
- Women
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Tampons as a self-administered collection method for the detection and quantification of genital HIV-1. / Webber, Mayris P.; Schoenbaum, Ellie; Farzadegan, Homayoon; Klein, Robert S.
In: AIDS, Vol. 15, No. 11, 27.07.2001, p. 1417-1420.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tampons as a self-administered collection method for the detection and quantification of genital HIV-1
AU - Webber, Mayris P.
AU - Schoenbaum, Ellie
AU - Farzadegan, Homayoon
AU - Klein, Robert S.
PY - 2001/7/27
Y1 - 2001/7/27
N2 - Objective: To assess the detection and quantitation of HIV-1 from tampon eluents in comparison with cervicovaginal lavage (CVL) and plasma specimens from the same women. Methods: Ninety-seven tampon, 105 CVL, and 104 plasma specimens from 105 HIV-1 seropositive women were analyzed using Version 3 of the Chiron bDNA assay, with sensitivity of 50 HIV-1 RNA copies/ml. Data analyses used McNemar's test, Wilcoxon signed rank test, and Mantel-Haenszel chi-squared and odds ratios with 95% confidence intervals to assess differences in proportions. Results: In women for whom both plasma and genital specimens were available, HIV-1 was detected less frequently in genital specimens: [tampons (33/97, 34%) and CVL (48/104, 46%)] than plasma specimens (86/104, 83%) (P< 0.001 for both plasma versus tampon and for plasma versus CVL). However, the proportion of genital specimens with detectable virus did not differ significantly by collection method (P = 0.14). Among women with detectable virus using both collection methods (n = 23), viral load was similar for tampon eluents (median, 355 copies/ml; range, 52-120898) and CVL specimens (median, 265 copies/ml; range, 61-35637; P = 0.88). Conclusion: Tampon eluent specimens are slightly less sensitive than CVL specimens in the detection of genital HIV-1, although quantification of viral load, when detectable by both methods, was similar.
AB - Objective: To assess the detection and quantitation of HIV-1 from tampon eluents in comparison with cervicovaginal lavage (CVL) and plasma specimens from the same women. Methods: Ninety-seven tampon, 105 CVL, and 104 plasma specimens from 105 HIV-1 seropositive women were analyzed using Version 3 of the Chiron bDNA assay, with sensitivity of 50 HIV-1 RNA copies/ml. Data analyses used McNemar's test, Wilcoxon signed rank test, and Mantel-Haenszel chi-squared and odds ratios with 95% confidence intervals to assess differences in proportions. Results: In women for whom both plasma and genital specimens were available, HIV-1 was detected less frequently in genital specimens: [tampons (33/97, 34%) and CVL (48/104, 46%)] than plasma specimens (86/104, 83%) (P< 0.001 for both plasma versus tampon and for plasma versus CVL). However, the proportion of genital specimens with detectable virus did not differ significantly by collection method (P = 0.14). Among women with detectable virus using both collection methods (n = 23), viral load was similar for tampon eluents (median, 355 copies/ml; range, 52-120898) and CVL specimens (median, 265 copies/ml; range, 61-35637; P = 0.88). Conclusion: Tampon eluent specimens are slightly less sensitive than CVL specimens in the detection of genital HIV-1, although quantification of viral load, when detectable by both methods, was similar.
KW - Cervicovaginal lavage
KW - Genital HIV-1
KW - Tampon
KW - Viral load
KW - Women
UR - http://www.scopus.com/inward/record.url?scp=0035958761&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035958761&partnerID=8YFLogxK
U2 - 10.1097/00002030-200107270-00011
DO - 10.1097/00002030-200107270-00011
M3 - Article
C2 - 11504963
AN - SCOPUS:0035958761
VL - 15
SP - 1417
EP - 1420
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 11
ER -