T15 PC binding monoclonal antibodies retain specificity when they switch from IgM to IgG

G. Spira, H. L. Aguila, Matthew D. Scharff

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The expression of some Ag-binding sites and their associated H and L chain V region genes are often dominated by one or another of the IgG subclasses. This is true of T15 anti-phosphorycholine (PC) response in which the dominant T15 H (V1, DFL11.6 J(H)1) and L (V(K)22, J(K)5) chain V regions are seldom found associated with the γ2b or γ2a regions in the circulation of BALB/c mice. In the present study we describe the characterization of γ2b, γ2a, and γ1 anti-PC antibodies obtained from IgM-producing hybridomas by Ig switching in culture. All switch variants retained the parental T15 idiotype, PC binding, and fine specificity. We thus conclude that the apparent in vivo restriction on the expressed T15 PC binding γ2 antibodies is not due to a conformational limitation on the expression of the anti-PC T15 binding site. Further, these studies confirm that in vitro switching can be used to generate mAb that are not readily available in vivo.

Original languageEnglish (US)
Pages (from-to)2675-2680
Number of pages6
JournalJournal of Immunology
Volume140
Issue number8
StatePublished - 1988
Externally publishedYes

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Antibody Specificity
Immunoglobulin M
Immunoglobulin G
Binding Sites
Monoclonal Antibodies
Hybridomas
Anti-Idiotypic Antibodies
Antibodies
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

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T15 PC binding monoclonal antibodies retain specificity when they switch from IgM to IgG. / Spira, G.; Aguila, H. L.; Scharff, Matthew D.

In: Journal of Immunology, Vol. 140, No. 8, 1988, p. 2675-2680.

Research output: Contribution to journalArticle

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AU - Spira, G.

AU - Aguila, H. L.

AU - Scharff, Matthew D.

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N2 - The expression of some Ag-binding sites and their associated H and L chain V region genes are often dominated by one or another of the IgG subclasses. This is true of T15 anti-phosphorycholine (PC) response in which the dominant T15 H (V1, DFL11.6 J(H)1) and L (V(K)22, J(K)5) chain V regions are seldom found associated with the γ2b or γ2a regions in the circulation of BALB/c mice. In the present study we describe the characterization of γ2b, γ2a, and γ1 anti-PC antibodies obtained from IgM-producing hybridomas by Ig switching in culture. All switch variants retained the parental T15 idiotype, PC binding, and fine specificity. We thus conclude that the apparent in vivo restriction on the expressed T15 PC binding γ2 antibodies is not due to a conformational limitation on the expression of the anti-PC T15 binding site. Further, these studies confirm that in vitro switching can be used to generate mAb that are not readily available in vivo.

AB - The expression of some Ag-binding sites and their associated H and L chain V region genes are often dominated by one or another of the IgG subclasses. This is true of T15 anti-phosphorycholine (PC) response in which the dominant T15 H (V1, DFL11.6 J(H)1) and L (V(K)22, J(K)5) chain V regions are seldom found associated with the γ2b or γ2a regions in the circulation of BALB/c mice. In the present study we describe the characterization of γ2b, γ2a, and γ1 anti-PC antibodies obtained from IgM-producing hybridomas by Ig switching in culture. All switch variants retained the parental T15 idiotype, PC binding, and fine specificity. We thus conclude that the apparent in vivo restriction on the expressed T15 PC binding γ2 antibodies is not due to a conformational limitation on the expression of the anti-PC T15 binding site. Further, these studies confirm that in vitro switching can be used to generate mAb that are not readily available in vivo.

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