T helper 1 cytokine mRNA is increased in spontaneously regressing primary melanomas

Michelle A. Lowes, G. Alex Bishop, Kerry Crotty, Ross St C Barnetson, Gary M. Halliday

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Spontaneous tumor regression, which is observed clinically and histologically in some primary melanomas, occurs in the absence of any effective therapy. It is probably immunologically mediated, because regressing melanomas are infiltrated with larger numbers of activated T cells, primarily CD4+, than nonregressing melanomas. To investigate the hypothesis that spontaneous regression of melanomas is caused by T-cell cytokine production, cytokine mRNA expression in 20 primary melanomas was examined using a noncompetitive, quantitative reverse-transcriptase polymerase chain reaction method. DNA standards were used to generate known numbers of molecules in each sample. Results were standardized to the internal control, glyceraldehyde-3-phosphate dehydrogenase, mRNA for CD38, lymphotoxin (TNF-α), and IL-2 were significantly elevated in the ten regressing melanomas compared to the ten nonregressing melanomas. IFN-υ mRNA was also elevated in regressing melanomas but failed to reach statistical significance. The Th2 cytokines IL-10 and IL-13 did not show differences in the regressing melanomas compared to nonregressing melanomas; neither did the pro-inflammatory cytokines IL-1α IL1β, IL-6, IL-8, and TNF-α, nor the growth factors, bFGF and TGF-β or GM-CSF. This study shows an association between Th1 cytokines and spontaneously regressing melanomas. Although we have not shown that these cytokines cause regression, these findings support our hypothesis that activated CD4+ T cells may mediate melanoma regression by secretion of Th1 cytokines.

Original languageEnglish (US)
Pages (from-to)914-919
Number of pages6
JournalJournal of Investigative Dermatology
Volume108
Issue number6
StatePublished - 1997
Externally publishedYes

Fingerprint

Melanoma
Cytokines
Messenger RNA
T-cells
Lymphotoxin-alpha
Glyceraldehyde-3-Phosphate Dehydrogenases
Interleukin-13
T-Lymphocytes
Polymerase chain reaction
RNA-Directed DNA Polymerase
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-8
Interleukin-1
Interleukin-10
Interleukin-2
Tumors
Interleukin-6
Intercellular Signaling Peptides and Proteins
Molecules
Reverse Transcriptase Polymerase Chain Reaction

Keywords

  • Interleukin-2
  • Lymphotoxin
  • Skin cancer
  • Tumor regression

ASJC Scopus subject areas

  • Dermatology

Cite this

Lowes, M. A., Bishop, G. A., Crotty, K., Barnetson, R. S. C., & Halliday, G. M. (1997). T helper 1 cytokine mRNA is increased in spontaneously regressing primary melanomas. Journal of Investigative Dermatology, 108(6), 914-919.

T helper 1 cytokine mRNA is increased in spontaneously regressing primary melanomas. / Lowes, Michelle A.; Bishop, G. Alex; Crotty, Kerry; Barnetson, Ross St C; Halliday, Gary M.

In: Journal of Investigative Dermatology, Vol. 108, No. 6, 1997, p. 914-919.

Research output: Contribution to journalArticle

Lowes, MA, Bishop, GA, Crotty, K, Barnetson, RSC & Halliday, GM 1997, 'T helper 1 cytokine mRNA is increased in spontaneously regressing primary melanomas', Journal of Investigative Dermatology, vol. 108, no. 6, pp. 914-919.
Lowes MA, Bishop GA, Crotty K, Barnetson RSC, Halliday GM. T helper 1 cytokine mRNA is increased in spontaneously regressing primary melanomas. Journal of Investigative Dermatology. 1997;108(6):914-919.
Lowes, Michelle A. ; Bishop, G. Alex ; Crotty, Kerry ; Barnetson, Ross St C ; Halliday, Gary M. / T helper 1 cytokine mRNA is increased in spontaneously regressing primary melanomas. In: Journal of Investigative Dermatology. 1997 ; Vol. 108, No. 6. pp. 914-919.
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