T-h-2 immunity and CD3+ CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope

Vivekanandhan Aravindhan, Sujatha Narayanan, Namasivayam Gautham, Vinayaka Prasad, P. Kannan, William R. Jacobs, Paranji R. Narayanan

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The genetic engineering of Mycobacterium bovis-bacillus Calmette-Guérin to express foreign epitopes is an attractive strategy in the field of epitope vaccines. We constructed an 'epitope-trap vector' with Mycobacterium tuberculosis chaperonin-10 as a carrier antigen and used it to express the HIV-1 principal neutralizing determinant epitope. We also identified a new chaperonin-10 promoter that was hyperexpressive compared with the heat shock protein-65 promoter. Splenocytes from recombinant bacillus Calmette-Guérin-immunized mice showed enhanced lymphocyte proliferation and interleukin-4 (but not interferon-γ) secretion. The recombinant bacillus Calmette-Guérin-immunized group also exhibited mild delayed-type hypersensitivity reaction and a high frequency of CD3+CD45RBlow-activated T cells, together with high titer of antiprincipal neutralizing determinant immunoglobulin G antibodies. Thus, this epitope delivery system induced strong epitope-specific T-h-2 polarization.

Original languageEnglish (US)
Pages (from-to)45-55
Number of pages11
JournalFEMS Immunology and Medical Microbiology
Volume47
Issue number1
DOIs
Publication statusPublished - Jun 1 2006

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Keywords

  • Chaperonin-10 (Cpn10) promoter
  • Cryptic epitopes
  • Epitope-trap vector
  • HIV-1 principal neutralizing determinant (PND)
  • Recombinant bacillus Calmette-Guérin (r-BCG)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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