T-h-2 immunity and CD3+ CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope

Vivekanandhan Aravindhan, Sujatha Narayanan, Namasivayam Gautham, Vinayaka R. Prasad, P. Kannan, William R. Jacobs, Paranji R. Narayanan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The genetic engineering of Mycobacterium bovis-bacillus Calmette-Guérin to express foreign epitopes is an attractive strategy in the field of epitope vaccines. We constructed an 'epitope-trap vector' with Mycobacterium tuberculosis chaperonin-10 as a carrier antigen and used it to express the HIV-1 principal neutralizing determinant epitope. We also identified a new chaperonin-10 promoter that was hyperexpressive compared with the heat shock protein-65 promoter. Splenocytes from recombinant bacillus Calmette-Guérin-immunized mice showed enhanced lymphocyte proliferation and interleukin-4 (but not interferon-γ) secretion. The recombinant bacillus Calmette-Guérin-immunized group also exhibited mild delayed-type hypersensitivity reaction and a high frequency of CD3+CD45RBlow-activated T cells, together with high titer of antiprincipal neutralizing determinant immunoglobulin G antibodies. Thus, this epitope delivery system induced strong epitope-specific T-h-2 polarization.

Original languageEnglish (US)
Pages (from-to)45-55
Number of pages11
JournalFEMS Immunology and Medical Microbiology
Volume47
Issue number1
DOIs
StatePublished - Jun 2006

Fingerprint

Bacillus
HIV-1
Epitopes
Immunity
T-Lymphocytes
Chaperonin 10
Genetic Engineering
Delayed Hypersensitivity
Mycobacterium bovis
Heat-Shock Proteins
Mycobacterium tuberculosis
Interleukin-4
Interferons
Vaccines
Immunoglobulin G
Lymphocytes
Antigens
Antibodies

Keywords

  • Chaperonin-10 (Cpn10) promoter
  • Cryptic epitopes
  • Epitope-trap vector
  • HIV-1 principal neutralizing determinant (PND)
  • Recombinant bacillus Calmette-Guérin (r-BCG)

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

T-h-2 immunity and CD3+ CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope. / Aravindhan, Vivekanandhan; Narayanan, Sujatha; Gautham, Namasivayam; Prasad, Vinayaka R.; Kannan, P.; Jacobs, William R.; Narayanan, Paranji R.

In: FEMS Immunology and Medical Microbiology, Vol. 47, No. 1, 06.2006, p. 45-55.

Research output: Contribution to journalArticle

Aravindhan, Vivekanandhan ; Narayanan, Sujatha ; Gautham, Namasivayam ; Prasad, Vinayaka R. ; Kannan, P. ; Jacobs, William R. ; Narayanan, Paranji R. / T-h-2 immunity and CD3+ CD45RBlow-activated T cells in mice immunized with recombinant bacillus Calmette-Guérin expressing HIV-1 principal neutralizing determinant epitope. In: FEMS Immunology and Medical Microbiology. 2006 ; Vol. 47, No. 1. pp. 45-55.
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