T cell receptor complexes containing FcεRIγ homodimers in lieu of CD3ζ and CD3η components

A novel isoform expressed on large granular lymphocytes

Shigeo Koyasu, Luciano D'Adamio, Antonio R N Arulanandam, Sheena Abraham, Linda K. Clayton, Ellis L. Reinherz

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

CD3ζ and CD3η form disulfide-linked homo- or heterodimers important in targeting partially assembled Tiα-β/CD3γδε T cell receptor (TCR) complexes to the cell surface and transducing stimulatory signals after antigen recognition. Here we identify a new TCR isoform expressed on splenic CD2+, CD3/Tiα-β+, CD4-, CD8-, CD16+, NK1.1+ mouse large granular lymphocytes (LGL), which are devoid of CD3ζ and CD3η proteins. The TCRs of this subset contain homodimers of the γ subunit of the high affinity receptor for IgE (FcεRIγ) in lieu of CD3ζ and/or CD3η proteins. The LGL display natural killer-like activity and are cytotoxic for B cell hybridomas producing anti-CD3ε and anti-CD16 monoclonal antibodies, demonstrating the signaling capacity of both TCR and CD16 in this cell type. These findings provide evidence for an additional level of complexity of TCR signal transduction isoforms in naturally occurring T cell subsets.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalJournal of Experimental Medicine
Volume175
Issue number1
StatePublished - 1992
Externally publishedYes

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T-Cell Antigen Receptor
Protein Isoforms
Lymphocytes
IgE Receptors
Hybridomas
T-Lymphocyte Subsets
Disulfides
Signal Transduction
Proteins
B-Lymphocytes
Monoclonal Antibodies
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Koyasu, S., D'Adamio, L., Arulanandam, A. R. N., Abraham, S., Clayton, L. K., & Reinherz, E. L. (1992). T cell receptor complexes containing FcεRIγ homodimers in lieu of CD3ζ and CD3η components: A novel isoform expressed on large granular lymphocytes. Journal of Experimental Medicine, 175(1), 203-209.

T cell receptor complexes containing FcεRIγ homodimers in lieu of CD3ζ and CD3η components : A novel isoform expressed on large granular lymphocytes. / Koyasu, Shigeo; D'Adamio, Luciano; Arulanandam, Antonio R N; Abraham, Sheena; Clayton, Linda K.; Reinherz, Ellis L.

In: Journal of Experimental Medicine, Vol. 175, No. 1, 1992, p. 203-209.

Research output: Contribution to journalArticle

Koyasu, S, D'Adamio, L, Arulanandam, ARN, Abraham, S, Clayton, LK & Reinherz, EL 1992, 'T cell receptor complexes containing FcεRIγ homodimers in lieu of CD3ζ and CD3η components: A novel isoform expressed on large granular lymphocytes', Journal of Experimental Medicine, vol. 175, no. 1, pp. 203-209.
Koyasu, Shigeo ; D'Adamio, Luciano ; Arulanandam, Antonio R N ; Abraham, Sheena ; Clayton, Linda K. ; Reinherz, Ellis L. / T cell receptor complexes containing FcεRIγ homodimers in lieu of CD3ζ and CD3η components : A novel isoform expressed on large granular lymphocytes. In: Journal of Experimental Medicine. 1992 ; Vol. 175, No. 1. pp. 203-209.
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