T cell clones specific for IgG2a of the a allotype: Direct evidence for presentation of endogenous antigen

E. Bikoff, B. K. Birshtein

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

T cell clones specific for IgG2a of the a allotype have been isolated from C57BL/6J mice. Antigenic determinants recognized by these clones were localized by using a panel of hybrid IgG2b-IgG2a myeloma proteins. These experiments provide evidence for two distinct antigenic sites, one located in a segment encompassing the hinge region and most of the C(H)2 domain, and the other in a segment spanning the C(H)3 domain and the C-terminal eight residues of the C(H)2 domain. As judged by their failure to respond in the presence of B6.C-H-2(bm12) spleen cells, all the clones recognize determinants created, in part, by the I-A(β) chain. A strong proliferative response was observed in the presence of spleen cells from several H-2b strains, including C3H.SW, A.BY, D1.LP, and BALB.B. Experiments testing reactivity directed toward spleen cells from appropriate allotype-congenic mouse strains demonstrated that this response was controlled by Igh-linked genes. These results clearly indicated 1) that Igh-1a-specific T cell clones are stimulated by endogenously synthesized IgG2a, and 2) these T cells recognize shared determinants expressed on IgG2a molecules of various strains. These experiments thus provide strong evidence for presentation of self antigens under normal physiological conditions.

Original languageEnglish (US)
Pages (from-to)28-34
Number of pages7
JournalJournal of Immunology
Volume137
Issue number1
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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