Synthetic Fab fragments that bind the HIV-1 gp41 heptad repeat regions

Yanyun Liu, Lauren K. Regula, Alex Stewart, Jonathan R. Lai

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Recent work has demonstrated that antibody phage display libraries containing restricted diversity in the complementarity determining regions (CDRs) can be used to target a wide variety of antigens with high affinity and specificity. In the most extreme case, antibodies whose combining sites are comprised of only two residues - tyrosine and serine - have been identified against several protein antigens. [F.A. Fellouse, B. Li, D.M. Compaan, A.A. Peden, S.G. Hymowitz, S.S. Sidhu, J. Mol. Biol. 348 (2005) 1153-1162.] Here, we report the isolation and characterization of antigen-binding fragments (Fabs) from such " minimalist" diversity synthetic antibody libraries that bind the heptad repeat regions of human immunodeficiency virus type 1 (HIV-1) gp41. We show that these Fabs are highly specific for the HIV-1 epitope and comparable in affinity to a single chain variable fragment (scFv) derived from a natural antibody repertoire that targets the same region. Since the heptad repeat regions of HIV-1 gp41 are required for viral entry, these Fabs have potential for use in therapeutic, research, or diagnostic applications.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume413
Issue number4
DOIs
StatePublished - Oct 7 2011

Keywords

  • Antibody engineering
  • HIV-1
  • Phage display

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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