Synthetic Fab fragments that bind the HIV-1 gp41 heptad repeat regions

Yanyun Liu, Lauren K. Regula, Alex Stewart, Jonathan R. Lai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Recent work has demonstrated that antibody phage display libraries containing restricted diversity in the complementarity determining regions (CDRs) can be used to target a wide variety of antigens with high affinity and specificity. In the most extreme case, antibodies whose combining sites are comprised of only two residues - tyrosine and serine - have been identified against several protein antigens. [F.A. Fellouse, B. Li, D.M. Compaan, A.A. Peden, S.G. Hymowitz, S.S. Sidhu, J. Mol. Biol. 348 (2005) 1153-1162.] Here, we report the isolation and characterization of antigen-binding fragments (Fabs) from such " minimalist" diversity synthetic antibody libraries that bind the heptad repeat regions of human immunodeficiency virus type 1 (HIV-1) gp41. We show that these Fabs are highly specific for the HIV-1 epitope and comparable in affinity to a single chain variable fragment (scFv) derived from a natural antibody repertoire that targets the same region. Since the heptad repeat regions of HIV-1 gp41 are required for viral entry, these Fabs have potential for use in therapeutic, research, or diagnostic applications.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume413
Issue number4
DOIs
StatePublished - Oct 7 2011

Fingerprint

Immunoglobulin Fab Fragments
Viruses
HIV-1
Antigens
Libraries
Antibodies
Antibody Diversity
Therapeutic Human Experimentation
Complementarity Determining Regions
Single-Chain Antibodies
Bacteriophages
Serine
Tyrosine
Epitopes
Binding Sites
Display devices
Proteins

Keywords

  • Antibody engineering
  • HIV-1
  • Phage display

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Synthetic Fab fragments that bind the HIV-1 gp41 heptad repeat regions. / Liu, Yanyun; Regula, Lauren K.; Stewart, Alex; Lai, Jonathan R.

In: Biochemical and Biophysical Research Communications, Vol. 413, No. 4, 07.10.2011, p. 611-615.

Research output: Contribution to journalArticle

Liu, Yanyun ; Regula, Lauren K. ; Stewart, Alex ; Lai, Jonathan R. / Synthetic Fab fragments that bind the HIV-1 gp41 heptad repeat regions. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 413, No. 4. pp. 611-615.
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