Synthesis of murine mammary tumor viral proteins in vitro

G. C. Sen, Janis Racevskis, N. H. Sarkar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The coding potential of murine mammary tumor viral genomic RNA was investigated by in vitro translation of various size classes of RNAs isolated from the virions. The major products of translation of full-size 35S polyadenylated virion RNA were gag-related polyproteins of 75,000, 105,000, and 180,000 daltons (P75, P105, and P180, respectively). Studies on the kinetics of translation of these three proteins established that they were synthesized independently and that the smaller proteins were not post-translational cleavage products of the larger proteins. Tryptic peptide mapping showed that almost all of the P75 sequences were contained within P105 and almost all of the P105 sequences were contained within P180. The syntheses of all three proteins were inhibited by m7GTP, indicating that they were translated from capped mRNA's. Although a 24S polyadenylated RNA had been identified as the intracellular mRNA for env precursor polyprotein, no such protein could be translated from the 24S polyadenylated RNA isolated from the virions. However, translation of a 14S size class of polyadenylated virion RNA yielded four prominent proteins of about 36,000, 23,000, 21,000, and 20,000 daltons. These proteins were unrelated to murine mammary tumor viral structural proteins, as suggested from tryptic peptide mapping and immunoprecipitation data. They might be the products of an as-yet-unidentified gene located near the 3' terminus of the murine mammary tumor viral genomic RNA.

Original languageEnglish (US)
Pages (from-to)963-975
Number of pages13
JournalJournal of Virology
Volume37
Issue number3
StatePublished - 1981
Externally publishedYes

Fingerprint

mammary neoplasms (animal)
viral proteins
Viral Proteins
Breast Neoplasms
Virion
synthesis
virion
mice
translation (genetics)
Messenger RNA
RNA
Proteins
Peptide Mapping
proteins
peptide mapping
Viral RNA
messenger RNA
env Gene Products
Viral Structural Proteins
gag Gene Products

ASJC Scopus subject areas

  • Immunology

Cite this

Synthesis of murine mammary tumor viral proteins in vitro. / Sen, G. C.; Racevskis, Janis; Sarkar, N. H.

In: Journal of Virology, Vol. 37, No. 3, 1981, p. 963-975.

Research output: Contribution to journalArticle

Sen, GC, Racevskis, J & Sarkar, NH 1981, 'Synthesis of murine mammary tumor viral proteins in vitro', Journal of Virology, vol. 37, no. 3, pp. 963-975.
Sen, G. C. ; Racevskis, Janis ; Sarkar, N. H. / Synthesis of murine mammary tumor viral proteins in vitro. In: Journal of Virology. 1981 ; Vol. 37, No. 3. pp. 963-975.
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