Synthesis of immunoglobulin by substrate attached mouse myeloma cells

A. Bargellesi, G. Damiani, W. M. Kuehl, Matthew D. Scharff

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cultured mouse myeloma cells grow in suspension and synthesize and secrete large amounts of immunoglobulin. Mouse myeloma cells which attach to a plastic substratum have been obtained by mutagenesis and subsequent selection. Normal mouse myeloma cells will also attach to plastic tissue culture dishes pre treated with poly L Lysine. The attached cells synthesize and secrete the same large amounts of immunoglobulin as the suspended cells.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalJournal of Cellular Physiology
Volume88
Issue number2
StatePublished - 1976

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Immunoglobulins
Tissue culture
Mutagenesis
Substrates
Lysine
Suspensions
Plastics

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Synthesis of immunoglobulin by substrate attached mouse myeloma cells. / Bargellesi, A.; Damiani, G.; Kuehl, W. M.; Scharff, Matthew D.

In: Journal of Cellular Physiology, Vol. 88, No. 2, 1976, p. 247-251.

Research output: Contribution to journalArticle

Bargellesi, A, Damiani, G, Kuehl, WM & Scharff, MD 1976, 'Synthesis of immunoglobulin by substrate attached mouse myeloma cells', Journal of Cellular Physiology, vol. 88, no. 2, pp. 247-251.
Bargellesi, A. ; Damiani, G. ; Kuehl, W. M. ; Scharff, Matthew D. / Synthesis of immunoglobulin by substrate attached mouse myeloma cells. In: Journal of Cellular Physiology. 1976 ; Vol. 88, No. 2. pp. 247-251.
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