Synthesis of folate- pegylated polyester nanoparticles encapsulating ixabepilone for targeting folate receptor overexpressing breast cancer cells

P. Siafaka, M. Betsiou, A. Tsolou, E. Angelou, Bogos Agianian, M. Koffa, S. Chaitidou, E. Karavas, K. Avgoustakis, D. Bikiaris

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The aim of this study was the preparation of novel polyester nanoparticles based on folic acid (FA)-functionalized poly(ethylene glycol)-poly(propylene succinate) (PEG-PPSu) copolymer and loaded with the new anticancer drug ixabepilone (IXA). These nanoparticles may serve as a more selective (targeted) treatment of breast cancer tumors overexpressing the folate receptor. The synthesized materials were characterized by (1)H-NMR, FTIR, XRD and DSC. The nanoparticles were prepared by a double emulsification and solvent evaporation method and characterized with regard to their morphology by scanning electron microscopy, drug loading with HPLC-UV and size by dynamic light scattering. An average size of 195 nm and satisfactory drug loading efficiency (3.5%) were observed. XRD data indicated that IXA was incorporated into nanoparticles in amorphous form. The nanoparticles exhibited sustained drug release properties in vitro. Based on in vitro cytotoxicity studies, the blank FA-PEG-PPSu nanoparticles were found to be non-toxic to the cells. Fluorescent nanoparticles were prepared by conjugating Rhodanine B to PEG-PPSu, and live cell, fluorescence, confocal microscopy was applied in order to demonstrate the ability of FA-PEG-PPSu nanoparticles to enter into human breast cancer cells expressing the folate receptor.

Original languageEnglish (US)
Pages (from-to)275
Number of pages1
JournalJournal of Materials Science: Materials in Medicine
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2015
Externally publishedYes

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Polyesters
Folic Acid
Nanoparticles
Cells
Breast Neoplasms
Ethylene Glycol
Polyethylene glycols
Polypropylenes
Pharmaceutical Preparations
Acids
Rhodanine
Emulsification
ixabepilone
Confocal microscopy
Dynamic light scattering
Fourier Transform Infrared Spectroscopy
Cytotoxicity
Fluorescence Microscopy
Confocal Microscopy
Electron Scanning Microscopy

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Synthesis of folate- pegylated polyester nanoparticles encapsulating ixabepilone for targeting folate receptor overexpressing breast cancer cells. / Siafaka, P.; Betsiou, M.; Tsolou, A.; Angelou, E.; Agianian, Bogos; Koffa, M.; Chaitidou, S.; Karavas, E.; Avgoustakis, K.; Bikiaris, D.

In: Journal of Materials Science: Materials in Medicine, Vol. 26, No. 12, 01.12.2015, p. 275.

Research output: Contribution to journalArticle

Siafaka, P. ; Betsiou, M. ; Tsolou, A. ; Angelou, E. ; Agianian, Bogos ; Koffa, M. ; Chaitidou, S. ; Karavas, E. ; Avgoustakis, K. ; Bikiaris, D. / Synthesis of folate- pegylated polyester nanoparticles encapsulating ixabepilone for targeting folate receptor overexpressing breast cancer cells. In: Journal of Materials Science: Materials in Medicine. 2015 ; Vol. 26, No. 12. pp. 275.
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