Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture

R. A. Murphy, Robert H. Singer, J. D. Saide, N. J. Pantazis, M. H. Blanchard, K. S. Byron, B. G. Arnason, M. Young

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Rat skeletal muscle cells and a cloned myogenic cell line synthesize and secrete in culture a molecule that is immunlogically and biologically indistinguishable from the active form of nerve growth factor (NGF) from mouse submandibular gland. This protein can be detected in medium conditioned by muscle cells both before and after fusion and in the soluble fraction of muscle cell homogenates. Chromatographic data slso reveal that the molecular properties of muscle cell NGF differ from those of the growth factor purified from mouse submandibular glands. Muscle cell NGF has a molecular weight between 140,000 and 160,000, whereas purified mouse gland NGF has a molecular weight of 26,000. The biologic function of muscle cell NGF is not known, although it could be that if plays some role relating to the association of nerves and muscle in vivo.

Original languageEnglish (US)
Pages (from-to)4496-4500
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number10
StatePublished - 1977
Externally publishedYes

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Nerve Growth Factor
Muscle Cells
Skeletal Muscle
Cell Culture Techniques
Molecular Weight
Submandibular Gland
Conditioned Culture Medium
Intercellular Signaling Peptides and Proteins
Cell Line
Muscles
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture. / Murphy, R. A.; Singer, Robert H.; Saide, J. D.; Pantazis, N. J.; Blanchard, M. H.; Byron, K. S.; Arnason, B. G.; Young, M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 74, No. 10, 1977, p. 4496-4500.

Research output: Contribution to journalArticle

Murphy, R. A. ; Singer, Robert H. ; Saide, J. D. ; Pantazis, N. J. ; Blanchard, M. H. ; Byron, K. S. ; Arnason, B. G. ; Young, M. / Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture. In: Proceedings of the National Academy of Sciences of the United States of America. 1977 ; Vol. 74, No. 10. pp. 4496-4500.
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AU - Blanchard, M. H.

AU - Byron, K. S.

AU - Arnason, B. G.

AU - Young, M.

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