Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture

R. A. Murphy, R. H. Singer, J. D. Saide, N. J. Pantazis, M. H. Blanchard, K. S. Byron, B. G. Arnason, M. Young

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Abstract

Rat skeletal muscle cells and a cloned myogenic cell line synthesize and secrete in culture a molecule that is immunlogically and biologically indistinguishable from the active form of nerve growth factor (NGF) from mouse submandibular gland. This protein can be detected in medium conditioned by muscle cells both before and after fusion and in the soluble fraction of muscle cell homogenates. Chromatographic data slso reveal that the molecular properties of muscle cell NGF differ from those of the growth factor purified from mouse submandibular glands. Muscle cell NGF has a molecular weight between 140,000 and 160,000, whereas purified mouse gland NGF has a molecular weight of 26,000. The biologic function of muscle cell NGF is not known, although it could be that if plays some role relating to the association of nerves and muscle in vivo.

Original languageEnglish (US)
Pages (from-to)4496-4500
Number of pages5
JournalUnknown Journal
Volume74
Issue number10
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

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    Murphy, R. A., Singer, R. H., Saide, J. D., Pantazis, N. J., Blanchard, M. H., Byron, K. S., Arnason, B. G., & Young, M. (1977). Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture. Unknown Journal, 74(10), 4496-4500. https://doi.org/10.1073/pnas.74.10.4496