Synthesis and processing of precursor polypeptides to murine mammary tumor virus structural proteins

Janis Racevskis, N. H. Sarkar

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Biosynthesis of murine mammary tumor virus (MuMTV) proteins was studied in the chronically MuMTV-infected epithelial cell line MuMT- 73 by using monospecific antisera to the major MuMTV core protein p27 and the major envelope glycoprotein gp47. In pulse-labeling experiments using [35S]methionine, monospecific antisera to p27 precipitated a 75,000-molecular-weight protein as the major intracellular component. Analysis of the same cellular extracts with monospecific antisera to gp47 revealed that the gp47 precursor was a 70,000-dalton protein. After chase periods, there was a loss of label from the precursors and a concomitant increase of labeled extracellular mature viral proteins. The glycoprotein precursor incorporated labeled glucosamine and seemed to be processed more rapidly than the p27 precursor. Considerable amounts of apparently nonvirion-associated gp47 and glycoprotein precursor could be detected in the extracellular culture fluid.

Original languageEnglish (US)
Pages (from-to)374-383
Number of pages10
JournalJournal of Virology
Volume25
Issue number1
StatePublished - 1978
Externally publishedYes

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Gammaretrovirus
Viral Structural Proteins
mammary neoplasms (animal)
structural proteins
Immune Sera
polypeptides
Glycoproteins
Breast Neoplasms
antiserum
glycoproteins
viruses
Peptides
synthesis
mice
Viral Core Proteins
Proteins
proteins
Glucosamine
Extracellular Fluid
Viral Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Synthesis and processing of precursor polypeptides to murine mammary tumor virus structural proteins. / Racevskis, Janis; Sarkar, N. H.

In: Journal of Virology, Vol. 25, No. 1, 1978, p. 374-383.

Research output: Contribution to journalArticle

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