Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria

Karthik Sekar, Roberto Rusconi, John T. Sauls, Tobias Fuhrer, Elad Noor, Jen Nguyen, Vicente I. Fernandez, Marieke F. Buffing, Michael Berney, Suckjoon Jun, Roman Stocker, Uwe Sauer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In natural environments, microbes are typically non-dividing and gauge when nutrients permit division. Current models are phenomenological and specific to nutrient-rich, exponentially growing cells, thus cannot predict the first division under limiting nutrient availability. To assess this regime, we supplied starving Escherichia coli with glucose pulses at increasing frequencies. Real-time metabolomics and microfluidic single-cell microscopy revealed unexpected, rapid protein, and nucleic acid synthesis already from minuscule glucose pulses in non-dividing cells. Additionally, the lag time to first division shortened as pulsing frequency increased. We pinpointed division timing and dependence on nutrient frequency to the changing abundance of the division protein FtsZ. A dynamic, mechanistic model quantitatively relates lag time to FtsZ synthesis from nutrient pulses and FtsZ protease-dependent degradation. Lag time changed in model-congruent manners, when we experimentally modulated the synthesis or degradation of FtsZ. Thus, limiting abundance of FtsZ can quantitatively predict timing of the first cell division.

Original languageEnglish (US)
Pages (from-to)e8623
JournalMolecular Systems Biology
Volume14
Issue number11
DOIs
StatePublished - Nov 5 2018

Fingerprint

Cell Division
Nutrients
Bacteria
cell division
Division
Degradation
Cells
Synthesis
Time Lag
Food
Predict
synthesis
degradation
bacteria
nutrients
Glucose
Timing
Cell
Limiting
glucose

Keywords

  • Escherichia coli
  • division
  • FtsZ
  • protein degradation
  • starvation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

Cite this

Sekar, K., Rusconi, R., Sauls, J. T., Fuhrer, T., Noor, E., Nguyen, J., ... Sauer, U. (2018). Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. Molecular Systems Biology, 14(11), e8623. https://doi.org/10.15252/msb.20188623

Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. / Sekar, Karthik; Rusconi, Roberto; Sauls, John T.; Fuhrer, Tobias; Noor, Elad; Nguyen, Jen; Fernandez, Vicente I.; Buffing, Marieke F.; Berney, Michael; Jun, Suckjoon; Stocker, Roman; Sauer, Uwe.

In: Molecular Systems Biology, Vol. 14, No. 11, 05.11.2018, p. e8623.

Research output: Contribution to journalArticle

Sekar, K, Rusconi, R, Sauls, JT, Fuhrer, T, Noor, E, Nguyen, J, Fernandez, VI, Buffing, MF, Berney, M, Jun, S, Stocker, R & Sauer, U 2018, 'Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria', Molecular Systems Biology, vol. 14, no. 11, pp. e8623. https://doi.org/10.15252/msb.20188623
Sekar, Karthik ; Rusconi, Roberto ; Sauls, John T. ; Fuhrer, Tobias ; Noor, Elad ; Nguyen, Jen ; Fernandez, Vicente I. ; Buffing, Marieke F. ; Berney, Michael ; Jun, Suckjoon ; Stocker, Roman ; Sauer, Uwe. / Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. In: Molecular Systems Biology. 2018 ; Vol. 14, No. 11. pp. e8623.
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