Synthesis and characterization of aptamer-targeted SNALPs for the delivery of siRNA

Samantha E. Wilner, Matthew Levy

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations

Abstract

Aptamers selected against cell surface receptors represent a unique set of ligands that can be used to target nanoparticles and other therapeutics to specific cell types. Here, we describe a method for using aptamers to deliver stable nucleic acid lipid particles (SNALPs) encapsulating small interfering RNA (siRNA) to cells in vitro. Using this method, we have demonstrated the ability of aptamer-conjugated SNALPs to achieve target-specific delivery and siRNA-mediated knockdown of a gene of interest. We also describe methods to characterize SNALP size, siRNA encapsulation efficiency, and aptamer conjugation efficiency.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages211-224
Number of pages14
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1380
ISSN (Print)1064-3745

Keywords

  • Aptamer
  • Liposome
  • SNALP
  • SiRNA
  • Targeted delivery

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Wilner, S. E., & Levy, M. (2016). Synthesis and characterization of aptamer-targeted SNALPs for the delivery of siRNA. In Methods in Molecular Biology (pp. 211-224). (Methods in Molecular Biology; Vol. 1380). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3197-2_18