Syntaxin-6 SNARE Involvement in Secretory and Endocytic Pathways of Cultured Pancreatic β-Cells

Regina Kuliawat, Elena Kalinina, Jason Bock, Lloyd D. Fricker, Timothy E. McGraw, Se Ryoung Kim, Jiayu Zhong, Richard Scheller, Peter Arvan

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

In pancreatic β-cells, the syntaxin 6 (Syn6) soluble N-ethylmaleimide-sensitive factor attachment protein receptor is distributed in the trans-Golgi network (TGN) (with spillover into immature secretory granules) and endosomes. A possible Syn6 requirement has been suggested in secretory granule biogenesis, but the role of Syn6 in live regulated secretory cells remains unexplored. We have created an ecdysone-inducible gene expression system in the INS-1 β-cell line and find that induced expression of a membrane-anchorless, cytosolic Syn6 (called Syn6t), but not full-length Syn6, causes a prominent defect in endosomal delivery to lysosomes, and the TGN, in these cells. The defect occurs downstream of the endosomal branchpoint involved in transferrin recycling, and upstream of the steady-state distribution of mannose 6-phosphate receptors. By contrast, neither acquisition of stimulus competence nor the ultimate size of β-granules is affected. Biosynthetic effects of dominant-interfering Syn6 seem limited to slowed intragranular processing to insulin (achieving normal levels within 2 h) and minor perturbation of sorting of newly synthesized lysosomal proenzymes. We conclude that expression of the Syn6t mutant slows a rate-limiting step in endosomal maturation but provides only modest and potentially indirect interference with regulated and constitutive secretory pathways, and in TGN sorting of lysosomal enzymes.

Original languageEnglish (US)
Pages (from-to)1690-1701
Number of pages12
JournalMolecular Biology of the Cell
Volume15
Issue number4
DOIs
StatePublished - Apr 2004

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Qa-SNARE Proteins
SNARE Proteins
Secretory Pathway
Cultured Cells
trans-Golgi Network
Secretory Vesicles
IGF Type 2 Receptor
Ecdysone
Enzyme Precursors
Endosomes
Recycling
Transferrin
Lysosomes
Mental Competency
Insulin
Gene Expression
Cell Line
Membranes
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Syntaxin-6 SNARE Involvement in Secretory and Endocytic Pathways of Cultured Pancreatic β-Cells. / Kuliawat, Regina; Kalinina, Elena; Bock, Jason; Fricker, Lloyd D.; McGraw, Timothy E.; Kim, Se Ryoung; Zhong, Jiayu; Scheller, Richard; Arvan, Peter.

In: Molecular Biology of the Cell, Vol. 15, No. 4, 04.2004, p. 1690-1701.

Research output: Contribution to journalArticle

Kuliawat, R, Kalinina, E, Bock, J, Fricker, LD, McGraw, TE, Kim, SR, Zhong, J, Scheller, R & Arvan, P 2004, 'Syntaxin-6 SNARE Involvement in Secretory and Endocytic Pathways of Cultured Pancreatic β-Cells', Molecular Biology of the Cell, vol. 15, no. 4, pp. 1690-1701. https://doi.org/10.1091/mbc.E03-08-0554
Kuliawat, Regina ; Kalinina, Elena ; Bock, Jason ; Fricker, Lloyd D. ; McGraw, Timothy E. ; Kim, Se Ryoung ; Zhong, Jiayu ; Scheller, Richard ; Arvan, Peter. / Syntaxin-6 SNARE Involvement in Secretory and Endocytic Pathways of Cultured Pancreatic β-Cells. In: Molecular Biology of the Cell. 2004 ; Vol. 15, No. 4. pp. 1690-1701.
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