Syndecan-1 regulates BMP signaling and dorso-ventral patterning of the ectoderm during early Xenopus development

Gonzalo H. Olivares, Héctor Carrasco, Francisco Aroca, Loreto Carvallo, Fabián Segovia, Juan Larraín

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Extracellular regulation of growth factor signaling is a key event for embryonic patterning. Heparan sulfate proteoglycans (HSPG) are among the molecules that regulate this signaling during embryonic development. Here we study the function of syndecan1 (Syn1), a cell-surface HSPG expressed in the non-neural ectoderm during early development of Xenopus embryos. Overexpression of Xenopus Syn1 (xSyn1) mRNA is sufficient to reduce BMP signaling, induce chordin expression and rescue dorso-ventral patterning in ventralized embryos. Experiments using chordin morpholinos established that xSyn1 mRNA can inhibit BMP signaling in the absence of chordin. Knockdown of xSyn1 resulted in a reduction of BMP signaling and expansion of the neural plate with the concomitant reduction of the non-neural ectoderm. Overexpression of xSyn1 mRNA in xSyn1 morphant embryos resulted in a biphasic effect, with BMP being inhibited at high concentrations and activated at low concentrations of xSyn1. Interestingly, the function of xSyn1 on dorso-ventral patterning and BMP signaling is specific for this HSPG. In summary, we report that xSyn1 regulates dorso-ventral patterning of the ectoderm through modulation of BMP signaling.

Original languageEnglish (US)
Pages (from-to)338-349
Number of pages12
JournalDevelopmental Biology
Volume329
Issue number2
DOIs
StatePublished - May 15 2009
Externally publishedYes

Fingerprint

Syndecan-1
Ectoderm
Xenopus
Heparan Sulfate Proteoglycans
Messenger RNA
Embryonic Development
Embryonic Structures
Neural Plate
Morpholinos
Intercellular Signaling Peptides and Proteins

Keywords

  • BMP signaling
  • Chordin
  • Dorso-ventral patterning
  • Ectoderm
  • HSPG
  • Syndecan1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Syndecan-1 regulates BMP signaling and dorso-ventral patterning of the ectoderm during early Xenopus development. / Olivares, Gonzalo H.; Carrasco, Héctor; Aroca, Francisco; Carvallo, Loreto; Segovia, Fabián; Larraín, Juan.

In: Developmental Biology, Vol. 329, No. 2, 15.05.2009, p. 338-349.

Research output: Contribution to journalArticle

Olivares, Gonzalo H. ; Carrasco, Héctor ; Aroca, Francisco ; Carvallo, Loreto ; Segovia, Fabián ; Larraín, Juan. / Syndecan-1 regulates BMP signaling and dorso-ventral patterning of the ectoderm during early Xenopus development. In: Developmental Biology. 2009 ; Vol. 329, No. 2. pp. 338-349.
@article{fa154fea206947e89a6c91deae0794f1,
title = "Syndecan-1 regulates BMP signaling and dorso-ventral patterning of the ectoderm during early Xenopus development",
abstract = "Extracellular regulation of growth factor signaling is a key event for embryonic patterning. Heparan sulfate proteoglycans (HSPG) are among the molecules that regulate this signaling during embryonic development. Here we study the function of syndecan1 (Syn1), a cell-surface HSPG expressed in the non-neural ectoderm during early development of Xenopus embryos. Overexpression of Xenopus Syn1 (xSyn1) mRNA is sufficient to reduce BMP signaling, induce chordin expression and rescue dorso-ventral patterning in ventralized embryos. Experiments using chordin morpholinos established that xSyn1 mRNA can inhibit BMP signaling in the absence of chordin. Knockdown of xSyn1 resulted in a reduction of BMP signaling and expansion of the neural plate with the concomitant reduction of the non-neural ectoderm. Overexpression of xSyn1 mRNA in xSyn1 morphant embryos resulted in a biphasic effect, with BMP being inhibited at high concentrations and activated at low concentrations of xSyn1. Interestingly, the function of xSyn1 on dorso-ventral patterning and BMP signaling is specific for this HSPG. In summary, we report that xSyn1 regulates dorso-ventral patterning of the ectoderm through modulation of BMP signaling.",
keywords = "BMP signaling, Chordin, Dorso-ventral patterning, Ectoderm, HSPG, Syndecan1",
author = "Olivares, {Gonzalo H.} and H{\'e}ctor Carrasco and Francisco Aroca and Loreto Carvallo and Fabi{\'a}n Segovia and Juan Larra{\'i}n",
year = "2009",
month = "5",
day = "15",
doi = "10.1016/j.ydbio.2009.03.007",
language = "English (US)",
volume = "329",
pages = "338--349",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Syndecan-1 regulates BMP signaling and dorso-ventral patterning of the ectoderm during early Xenopus development

AU - Olivares, Gonzalo H.

AU - Carrasco, Héctor

AU - Aroca, Francisco

AU - Carvallo, Loreto

AU - Segovia, Fabián

AU - Larraín, Juan

PY - 2009/5/15

Y1 - 2009/5/15

N2 - Extracellular regulation of growth factor signaling is a key event for embryonic patterning. Heparan sulfate proteoglycans (HSPG) are among the molecules that regulate this signaling during embryonic development. Here we study the function of syndecan1 (Syn1), a cell-surface HSPG expressed in the non-neural ectoderm during early development of Xenopus embryos. Overexpression of Xenopus Syn1 (xSyn1) mRNA is sufficient to reduce BMP signaling, induce chordin expression and rescue dorso-ventral patterning in ventralized embryos. Experiments using chordin morpholinos established that xSyn1 mRNA can inhibit BMP signaling in the absence of chordin. Knockdown of xSyn1 resulted in a reduction of BMP signaling and expansion of the neural plate with the concomitant reduction of the non-neural ectoderm. Overexpression of xSyn1 mRNA in xSyn1 morphant embryos resulted in a biphasic effect, with BMP being inhibited at high concentrations and activated at low concentrations of xSyn1. Interestingly, the function of xSyn1 on dorso-ventral patterning and BMP signaling is specific for this HSPG. In summary, we report that xSyn1 regulates dorso-ventral patterning of the ectoderm through modulation of BMP signaling.

AB - Extracellular regulation of growth factor signaling is a key event for embryonic patterning. Heparan sulfate proteoglycans (HSPG) are among the molecules that regulate this signaling during embryonic development. Here we study the function of syndecan1 (Syn1), a cell-surface HSPG expressed in the non-neural ectoderm during early development of Xenopus embryos. Overexpression of Xenopus Syn1 (xSyn1) mRNA is sufficient to reduce BMP signaling, induce chordin expression and rescue dorso-ventral patterning in ventralized embryos. Experiments using chordin morpholinos established that xSyn1 mRNA can inhibit BMP signaling in the absence of chordin. Knockdown of xSyn1 resulted in a reduction of BMP signaling and expansion of the neural plate with the concomitant reduction of the non-neural ectoderm. Overexpression of xSyn1 mRNA in xSyn1 morphant embryos resulted in a biphasic effect, with BMP being inhibited at high concentrations and activated at low concentrations of xSyn1. Interestingly, the function of xSyn1 on dorso-ventral patterning and BMP signaling is specific for this HSPG. In summary, we report that xSyn1 regulates dorso-ventral patterning of the ectoderm through modulation of BMP signaling.

KW - BMP signaling

KW - Chordin

KW - Dorso-ventral patterning

KW - Ectoderm

KW - HSPG

KW - Syndecan1

UR - http://www.scopus.com/inward/record.url?scp=67349158348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349158348&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2009.03.007

DO - 10.1016/j.ydbio.2009.03.007

M3 - Article

C2 - 19303002

AN - SCOPUS:67349158348

VL - 329

SP - 338

EP - 349

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -