Synaptic corelease of ATP and GABA in cultured spinal neurons

Young-Hwan Jo, Rémy Schlichter

Research output: Contribution to journalArticle

277 Citations (Scopus)

Abstract

In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.

Original languageEnglish (US)
Pages (from-to)241-245
Number of pages5
JournalNature Neuroscience
Volume2
Issue number3
DOIs
StatePublished - Mar 1999
Externally publishedYes

Fingerprint

gamma-Aminobutyric Acid
Neurotransmitter Agents
Adenosine Triphosphate
Neurons
Nociceptive Pain
Posterior Horn Cells
Inhibitory Postsynaptic Potentials
Hyperalgesia
Neuralgia
Neuropeptides
Adenosine
Glutamic Acid
Spinal Cord Dorsal Horn

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Synaptic corelease of ATP and GABA in cultured spinal neurons. / Jo, Young-Hwan; Schlichter, Rémy.

In: Nature Neuroscience, Vol. 2, No. 3, 03.1999, p. 241-245.

Research output: Contribution to journalArticle

Jo, Young-Hwan ; Schlichter, Rémy. / Synaptic corelease of ATP and GABA in cultured spinal neurons. In: Nature Neuroscience. 1999 ; Vol. 2, No. 3. pp. 241-245.
@article{60c121a0507d43c98e6dbac9a2a1296f,
title = "Synaptic corelease of ATP and GABA in cultured spinal neurons",
abstract = "In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.",
author = "Young-Hwan Jo and R{\'e}my Schlichter",
year = "1999",
month = "3",
doi = "10.1038/6344",
language = "English (US)",
volume = "2",
pages = "241--245",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Synaptic corelease of ATP and GABA in cultured spinal neurons

AU - Jo, Young-Hwan

AU - Schlichter, Rémy

PY - 1999/3

Y1 - 1999/3

N2 - In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.

AB - In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.

UR - http://www.scopus.com/inward/record.url?scp=0033363371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033363371&partnerID=8YFLogxK

U2 - 10.1038/6344

DO - 10.1038/6344

M3 - Article

C2 - 10195216

AN - SCOPUS:0033363371

VL - 2

SP - 241

EP - 245

JO - Nature Neuroscience

JF - Nature Neuroscience

SN - 1097-6256

IS - 3

ER -