Synaptic corelease of ATP and GABA in cultured spinal neurons

Young Hwan Jo; Rémy Schlichter

doi:10.1038/6344

Synaptic corelease of ATP and GABA in cultured spinal neurons

Young Hwan Jo, Rémy Schlichter

Research output: Contribution to journal › Article › peer-review

312 Scopus citations

Abstract

In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.

Original language	English (US)
Pages (from-to)	241-245
Number of pages	5
Journal	Nature Neuroscience
Volume	2
Issue number	3
DOIs	https://doi.org/10.1038/6344
State	Published - Mar 1999
Externally published	Yes

ASJC Scopus subject areas

General Neuroscience

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10.1038/6344

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title = "Synaptic corelease of ATP and GABA in cultured spinal neurons",

abstract = "In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.",

author = "Jo, {Young Hwan} and R{\'e}my Schlichter",

note = "Funding Information: We thank Catherine Moreau and Madeleine Roth for technical assistance and Sylvain Hugel for help with some of the experiments. This work was supported by a grant from the UPSA Pain Institute. We acknowledge further support from the Universit{\'e} Louis Pasteur and the Centre National de la Recherche Scientifique (CNRS).",

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N1 - Funding Information: We thank Catherine Moreau and Madeleine Roth for technical assistance and Sylvain Hugel for help with some of the experiments. This work was supported by a grant from the UPSA Pain Institute. We acknowledge further support from the Université Louis Pasteur and the Centre National de la Recherche Scientifique (CNRS).

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N2 - In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.

AB - In the spinal dorsal horn (DH), transmission and modulation of peripheral nociceptive (pain-inducing) messages involve classical neurotransmitters and neuropeptides. We show that approximately half of DH neurons use ATP as a fast excitatory neurotransmitter acting at ionotropic P2X postsynaptic receptors. ATP was not codetected with glutamate but was coreleased with the inhibitory neurotransmitter GABA. Moreover, adenosine, probably generated by extracellular metabolism of ATP, finely tuned GABAergic inhibitory postsynaptic currents. Differential modulation of excitatory versus inhibitory components of this mixed cotransmission may help to explain changes in sensory message processing in the DH during mechanical hyperalgesia and neuropathic pain.

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Synaptic corelease of ATP and GABA in cultured spinal neurons

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