Syk tyrosine kinase is required for immunoreceptor tyrosine activation motif-dependent actin assembly

Dianne Cox, Peter Chang, Tomohiro Kurosaki, Steven Greenberg

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Clustering of several multisubunit receptors on hematopoetic cells results in a signaling cascade involving the phosphorylation of immunoreceptor tyrosine activation motifs, or 'ITAMs,' and actin polymerization. Recent experiments indicate that direct clustering of the ITAM-binding protein, p72(syk) (Syk), is capable of transmitting a phagocytic signal in COS cells (Greenberg, S., Chang, P., Wang, D., Xavier, R., and Seed, B. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 1103-1107). However, the possibility of redundant signaling pathways makes it difficult to test the requirement for Syk in ITAM-dependent actin polymerization in hematopoetic cells. We developed a model system to study ITAM-dependent actin assembly. DT40 lymphocytes were transfected with fusion proteins encoding the transmembrane and cytosolic domains of the ITAM-containing γ subunit of Fc receptors. Clustering the γ-containing fusion proteins with IgG-coated erythrocytes triggered submembranous actin assembly. This response depended on an intact ITAM, was absent in cell lines that had been engineered to lack Syk, and was augmented in cell lines that stably overexpressed Syk. These experiments demonstrate an absolute requirement for Syk tyrosine kinase in ITAM-dependent actin assembly in transfected lymphocytes.

Original languageEnglish (US)
Pages (from-to)16597-16602
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number28
DOIs
StatePublished - 1996
Externally publishedYes

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Protein-Tyrosine Kinases
Tyrosine
Actins
Chemical activation
Cluster Analysis
Lymphocytes
Polymerization
Fusion reactions
Cells
Cell Line
Phosphorylation
Fc Receptors
COS Cells
Carrier Proteins
Proteins
Immunoglobulin G
Erythrocytes
Experiments
Syk Kinase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Syk tyrosine kinase is required for immunoreceptor tyrosine activation motif-dependent actin assembly. / Cox, Dianne; Chang, Peter; Kurosaki, Tomohiro; Greenberg, Steven.

In: Journal of Biological Chemistry, Vol. 271, No. 28, 1996, p. 16597-16602.

Research output: Contribution to journalArticle

Cox, Dianne ; Chang, Peter ; Kurosaki, Tomohiro ; Greenberg, Steven. / Syk tyrosine kinase is required for immunoreceptor tyrosine activation motif-dependent actin assembly. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 28. pp. 16597-16602.
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