Sustained nitric oxide-releasing nanoparticles interfere with methicillinresistant staphylococcus aureus adhesion and biofilm formation in a rat central venous catheter model

Mircea Radu Mihu, Vitor Cabral, Rodney Pattabhi, Moses T. Tar, Kelvin Davies, Adam J. Friedman, Luis R. Martinez, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Staphylococcus aureus is frequently isolated in the setting of infections of indwelling medical devices, which are mediated by the microbe's ability to form biofilms on a variety of surfaces. Biofilm-embedded bacteria are more resistant to antimicrobial agents than their planktonic counterparts and often cause chronic infections and sepsis, particularly in patients with prolonged hospitalizations. In this study, we demonstrate that sustained nitric oxide-releasing nanoparticles (NO-np) interfere with S. aureus adhesion and prevent biofilm formation on a rat central venous catheter (CVC) model of infection. Confocal and scanning electron microscopy showed that NO-np-treated staphylococcal biofilms displayed considerably reduced thicknesses and bacterial numbers compared to those of control biofilms in vitro and in vivo, respectively. Although both phenotypes, planktonic and biofilmassociated staphylococci, of multiple clinical strains were susceptible to NO-np, bacteria within biofilms were more resistant to killing than their planktonic counterparts. Furthermore, chitosan, a biopolymer found in the exoskeleton of crustaceans and structurally integrated into the nanoparticles, seems to add considerable antimicrobial activity to the technology. Our findings suggest promising development and translational potential of NO-np for use as a prophylactic or therapeutic against bacterial biofilms on CVCs and other medical devices.

Original languageEnglish (US)
Article numbere02020
JournalAntimicrobial Agents and Chemotherapy
Volume61
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Central Venous Catheters
Biofilms
Nanoparticles
Staphylococcus aureus
Nitric Oxide
Infection
Bacteria
Equipment and Supplies
Biopolymers
Chitosan
Anti-Infective Agents
Staphylococcus
Electron Scanning Microscopy
Sepsis
Hospitalization
Technology
Phenotype

Keywords

  • Antimicrobials
  • Biofilms
  • Nanoparticles
  • Nitric oxide
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Sustained nitric oxide-releasing nanoparticles interfere with methicillinresistant staphylococcus aureus adhesion and biofilm formation in a rat central venous catheter model. / Mihu, Mircea Radu; Cabral, Vitor; Pattabhi, Rodney; Tar, Moses T.; Davies, Kelvin; Friedman, Adam J.; Martinez, Luis R.; Nosanchuk, Joshua D.

In: Antimicrobial Agents and Chemotherapy, Vol. 61, No. 1, e02020, 01.01.2017.

Research output: Contribution to journalArticle

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